The aim of this study was to assess the risk of pesticide contamination in aquaculture and its impact on fishery products. We conducted an assessment of 300 samples collected from nine regions in South Korea, including various types of seafood, such as freshwater fish, marine fish, crustaceans, and shellfish. Pesticide residues in seafood were analyzed using GC-MS/MS after sample preparation using a modified QuEChERS method, revealing the presence of eight pesticides (4,4\'-DDE, 4,4\'-DDT, boscalid, isoprothiolane, oxadiazon, pendimethalin, thifluzamide, and trifluralin) across seven fish species (carp, far eastern catfish, crucian carp, eel, Chinese muddy loach, mirror carp, and sea bass). Following the grouping of DDE with DDT, a risk assessment of fishery products was conducted. After the estimated daily intake (EDI) of fish was calculated and compared with the acceptable daily intake (ADI), the health risk index (HI, %ADI) of the detected pesticides was evaluated and found to be 1.07% or lower. The results suggest that the consumption of domestically farmed fish products in South Korea poses minimal health risks associated with pesticide residues.

The mi-iuy croaker Miichthys miiuy has immense commercial value in the Republic of Korea. The red drum Sciaenops ocellatus is widely produced by aquaculture, although its price is approximately 25% that of M. miiuy. S. ocellatus has black spots on its tail, enabling it to be distinguished from M. miiuy based on appearance. However, identifying S. ocellatus after simple processing steps, such as skin removal and dicing, is difficult. Certain traders misrepresent and sell S. ocellatus as M. miiuy or cultured M. miiuy for illegal economical gain. Therefore, an accurate and rapid identification method is required to distinguish between M. miiuy and S. ocellatus in the field. Here, a method for rapid field identification was developed based on species-specific primers using a portable ultra-fast PCR instrument. The ultra-fast real-time PCR method can complete the entire analytical procedure, including DNA isolation, amplification, and detection, within 30 min, thus maintaining the accuracy of identifying M. miiuy and S. ocellatus products on site. Forty-nine commercial products were tested, and all samples were successfully identified. Thus, the developed method is rapid, efficient tool for ensuring consumer protection.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s10068-021-00954-4.

Sustainably feeding a growing human population is one of the greatest food system challenges of the 21st century. Seafood plays a vital role in supporting human wellbeing, by providing bioavailable and nutrient-dense animal-source food. In Thailand, seafood demand is increasing, and wild capture fishery yields have plateaued, due to oceanic ecosystem degradation and fishery stock exploitation. In this study, we investigated the supply trend of fishery products and subsequent seafood-derived nutrient availability over the last decade. In addition, we explored the possibility of predicting seafood availability and consumption levels, including adherence to Thailand\'s national food guide and global dietary recommendations for sustainable seafood consumption. Our findings indicate that, at national-level, fishery products supplied between 19% and 35% of the Thai populations recommended dietary protein intake, 4-6% of calcium, 6-11% of iron, and 2-4% of zinc from 1995 to 2015. Nevertheless, our research also reports that if Thailand\'s wild-caught seafood production were to decrease by 13%, as is highly likely, by 2030, the country might face a per capita supply deficit of fish and shellfish to meet healthy and sustainable dietary recommendations (28-30 g/day), let alone the current Thai average intake (32 g/day). Although a 1% per year increase in aquaculture production might bridge this supply gap, policymakers and relevant fishery stakeholders must consider the long-term environmental impacts of such an approach in Thailand.

The measurement and analysis of fifty-two elements by quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS) and direct mercury analysis were applied to origin discrimination of Italian traditional cuttlefish (Chioggia, Venice lagoon) from Mediterranean and Atlantic samples. A total 68 specimens were analyzed in triplicates to generate 204 mass spectra profiles which were statistically processed by different chemometric techniques. Loading weights from principal component analysis as input for linear discriminant analysis (LW-LDA), stepwise-LDA (S-LDA) and variable influence of projection-partial least square discriminant analysis (VIP-PLS-DA) were used to classify samples while retaining the lowest possible number of key variables. VIP-PLS-DA was found to be the best variable selection-discriminant tool combo since the selected Na-Co-B-K-Cd-V-U-Rb-Ni-Ba-Cu-As-Sr-Mn-Mo-Li-Ca-Mg-Se-Bi-Cs-P-Y elemental pattern allowed the samples to be classified with 100% sensitivity, specificity and accuracy.

Total mercury (Hg) concentration and stable isotope ratios (δ13C and δ15N) were used as chemical tracers in two crustaceans and four fish species that are targets of subsistence fishery in the inner estuary of the Paraíba do Sul River. This fluvial environment in southeast Brazil has a large historical utilization of Hg. δ15N was a better predictor of Hg concentration in the muscle (edible part) than δ13C; i.e., the Hg concentration was more closely related to the trophic position of species than species\' trophic habitat. Consumers at higher trophic levels had higher Hg concentrations. The Hg concentration was below the maximum permissible limits established by the Brazilian Government in fishery products, and there are no human risks related to its consumption.

Hydrogen peroxide (H2O2) has been reported to be used for the illegal treatment of fishery products in order to obtain \"fake\" freshness. Residues of H2O2 in food may be of toxicology concern. In this study, a nonenzymatic sensor was developed based on Fe@PCN-224 metal-organic frameworks wrapped by Nafion to detect H2O2 concentration. The hybrid structure of Fe@PCN-224 was fabricated by incorporated free FeIII ions into the center of PCN-224, which was ultra-stable due to the strong interactions between Zr6 and the carboxyl group. Scanning electron spectroscopy images exhibited that Nafion sheets crossed together on the surface of Fe@PCN-224 nanoparticles to form a hierarchical and coherent structure for efficient electron transfer. Electrochemical investigations showed that the Fe@PCN-224/Nafion/GCE possessed good linearity from 2 to 13,000 μM (including four orders of magnitude), low detection limits (0.7 μM), high stability in continuous monitoring (current remained nearly stable over 2300 s) and in long-term measurement (current decreased 3.4% for 30 days). The prepared nanohybrid modified electrode was effectively applied to H2O2 detection in three different fishery products. The results were comparable to those measured using photometrical methods. The developed electrochemical method has a great potential in detecting the illegal management of fishery products with H2O2.

Fishery products are the main source of dietary n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). Following the European Commission\'s request to address the risks and benefits of seafood consumption, and taking into account the great variability of nutrient and contaminant levels in fishery products, the present work aims to estimate the n-3 LC-PUFA provided per serving of selected fishes, shrimps and mollusks that are commonly consumed in Spain. This would enable the establishment of a risk-benefit analysis of fish consumption and provide recommendations for fish intake to comply with nutritional guidelines of n-3 LC-PUFA intake. We confirmed high variation in the pattern and contents of fatty acids for different species. n-6 PUFA were minor fatty acids, whereas palmitic (C16:0), oleic (C18:1 n-9), and mainly eicosapentaenoic (C20:5 n-3) and docosahexaenoic (C22:6 n-3) acids were the major fatty acids in the sample. Therefore, consumption of 2-3 servings per week of a variety of fishery products may contribute to compliance with the recommended daily n-3 LC-PUFA intake while maintaining an adequate balance to avoid contaminant-derived potential risks (metals and others). Taking the fatty acid content of fishery products described in this study into consideration, it is advisable to include one serving of fatty fish per week in order to meet recommended n-3 LC-PUFA levels.

Herein, an analytical method was developed for simultaneous determination of 12 anthelmintics (closantel, niclosamide, nitroxynil, rafoxanide, cymiazole, fluazuron, levamisole, morantel, praziquantel, pyrantel, thiophanate, and trichlorfon) in fishery products (eel, flatfish, and shrimp) using liquid-liquid extraction coupled with liquid chromatography-tandem mass spectrometry. A reversed-phase analytical column was then used to separate the analytes from various matrices. Linear matrix-matched calibration curves were generated with coefficients of determination ≥ 0.9935. Recovery rates at three spiking levels (5, 10, and 20 µg/kg) ranged between 61.58% and 119.37% with relative standard deviations ≤ 19.05%. Limits of detection were in the range of 0.3-1.6 μg/kg, whereas limits of quantification ranged between 1.0 and 5.0 μg/kg. The matrix effect was moderate with values ranging from -99.47% to 51.98%. Matrices procured from large markets tested negative for the 12 anthelmintics. The developed method proved amenable to real sample testing and can be used for simultaneous determination of target analytes in aquatic products.

The presence of parasites, including Anisakis spp. larvae in fishery products jeopardizes their safety and quality, which is a matter of concern and a challenge for official authorities, as well as for food businesses operators, and a potential health risk to consumers. In the last ten years, a total of 2367 samples from different seafood products, whole fish (n = 1022), fish steaks (n = 417), fish fillets (n = 46), roes (n = 11), canned fish fillets (n = 35), fish fingers (n = 705), and ready-made Japanese dishes (n = 131) were analysed at the Laboratory of Pathology of Aquatic Animals of IPMA. From the fishery products analysed, a total of 4643 visible Anisakis larvae were collected from eighteen different species of whole fish and there were two cases with numerous larvae, although live larvae were only found in fresh fish. Anisakis larvae (n = 523) were collected from fish steaks, (n = 20) from frozen fish fillets, (n = 7) from five different brands of fish fingers, (n = 2) from two cans of cod fillets and numerous viable larvae were collected from the serosae of European hake fresh roes. The great majority of the larvae were identified as L3 Anisakis larvae. Plerocercoids of Gymnorhynchus gigas and Hepatoxylon trichiuri and the myxosporean, Kudoa spp. were also observed and led to the withdrawal of the fish from the supply chain. No Anisakis larvae were collected from the farmed fish as well as from the ready-made Japanese dishes. However, Kudoa hexapunctata spores were observed in the tuna muscle from one sashimi meal. The data shows that fresh fish with live Anisakis larvae have been marketed. Educational strategies concerning parasites were implemented with workshops, \"Parasites in Fishery Products\" to train and instruct professionals within the fish supply chain and training activities for target groups, including children, were promoted, to raise awareness of this issue. One of the most relevant outcomes obtained was the improvement of implementation of parasite identification techniques in the industry, including private labs, and consequently: (1) decrease in the parasitological analysis number of common parasites and (2) reduction in the analysis of anatomical structures confused with parasites requested to IPMA\'s Laboratory of Pathology. In addition, the practice of freezing trash fish used to feed farmed fish, and the placement of nets at the water entry points of the earth ponds, in order to avoid wild fish introduction, were simple and important measures that were implemented to prevent Anisakis and other parasites entering fish farms. The two complementary parts of this work, laboratorial activities used to support the fish supply chain, and the educational strategies concerning parasites, have made an essential contribution towards the availability of data for assessing and minimizing the level of risk associated with fish consumption, and have particularly increased and improved awareness, regarding foodborne parasites.

Palytoxin is an emergent toxin in Europe and one of the most toxic substances know to date. The toxin disrupts the physiological functioning of the Na+/K+-ATPase converting the enzyme in a permeant cation channel. Human intoxications by PLTX after consumption of contaminated fishery products are a serious health issue and can be fatal. Several reports have previously investigated the oral and intraperitoneal toxicity of PLTX in mice. However, in all cases short observation periods (24 and 48 h) after toxin administration were evaluated. In this work, single oral or intraperitoneal doses of PLTX were administered to healthy mice and surviving animals were followed up for 96 h. The data obtained here allowed us to calculate the oral and intraperitoneal lethal doses 50 (LD50) which were in the range of the values previously described. Surprisingly, the oral NOAEL for PLTX was more than 10 times lower than that previously described, a fact that indicates the need for the reevaluation of the levels of the toxin in edible fishery products.