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Abstract：
Herein, an analytical method was developed for simultaneous determination of 12 anthelmintics (closantel, niclosamide, nitroxynil, rafoxanide, cymiazole, fluazuron, levamisole, morantel, praziquantel, pyrantel, thiophanate, and trichlorfon) in fishery products (eel, flatfish, and shrimp) using liquid-liquid extraction coupled with liquid chromatography-tandem mass spectrometry. A reversed-phase analytical column was then used to separate the analytes from various matrices. Linear matrix-matched calibration curves were generated with coefficients of determination ≥ 0.9935. Recovery rates at three spiking levels (5, 10, and 20 µg/kg) ranged between 61.58% and 119.37% with relative standard deviations ≤ 19.05%. Limits of detection were in the range of 0.3-1.6 μg/kg, whereas limits of quantification ranged between 1.0 and 5.0 μg/kg. The matrix effect was moderate with values ranging from -99.47% to 51.98%. Matrices procured from large markets tested negative for the 12 anthelmintics. The developed method proved amenable to real sample testing and can be used for simultaneous determination of target analytes in aquatic products.
摘要：
在这里，开发了一种同时测定12种驱虫药(closantel，氯硝柳胺,硝氧胺,rafoxanide,咪唑,氟草隆,左旋咪唑,morantel,吡喹酮,pyrantel,托布津,和敌百虫)在渔业产品(鳗鱼，比目鱼,和虾）使用液-液萃取结合液相色谱-串联质谱法。然后使用反相分析柱从各种基质中分离分析物。生成线性矩阵匹配的校准曲线，测定系数≥0.9935。三个加标水平（5、10和20µg/kg）的回收率在61.58％至119.37％之间，相对标准偏差≤19.05％。检测范围为0.3-1.6μg/kg，而定量限范围为1.0至5.0μg/kg。基体效应是中等的,值在-99.47%至51.98%的范围内。从大型市场采购的基质对12种驱虫药测试呈阴性。所开发的方法被证明适用于实际样品测试，可用于同时测定水产品中的目标分析物。
