先前已发现伯氏疏螺旋体SpoVG蛋白是DNA和RNA结合蛋白。为了帮助阐明配体基序,对许多RNA的亲和力,ssDNA,和dsDNA进行测量和比较。研究中使用的基因座是spoVG,glpFKD,erpAB,bb0242,flaB,和OspAB,特别关注mRNA的未翻译5'部分。进行结合和竞争测定得出,spoVGmRNA的5'末端具有最高的亲和力,而观察到的最低亲和力是flaBmRNA的5'末端。spoVGRNA和ssDNA序列的诱变研究表明,spoVG-核酸复合物的形成不完全依赖于序列或结构。此外,在ssDNA中将尿嘧啶交换为胸腺嘧啶不会影响蛋白质-核酸复合物的形成。
The Borrelia burgdorferi SpoVG protein has previously been found to be a DNA- and RNA-binding protein. To aid in the elucidation of ligand motifs, affinities for numerous RNAs, ssDNAs, and dsDNAs were measured and compared. The loci used in the study were spoVG, glpFKD, erpAB, bb0242, flaB, and ospAB, with particular focus on the untranslated 5\' portion of the mRNAs. Performing binding and competition assays yielded that the 5\' end of spoVG mRNA had the highest affinity while the lowest observed affinity was to the 5\' end of flaB mRNA. Mutagenesis studies of spoVG RNA and ssDNA sequences suggested that the formation of SpoVG-nucleic acid complexes are not entirely dependent on either sequence or structure. Additionally, exchanging uracil for thymine in ssDNAs did not affect protein-nucleic acid complex formation.