%0 Journal Article
%T Protocol to identify DNA-binding proteins recognizing nucleotide repeat dsDNAs.
%A Huang Z
%A Zhou Y
%A Liu Y
%A Wang J
%J STAR Protoc
%V 5
%N 2
%D 2024 Jun 21
%M 38613779
暂无%R 10.1016/j.xpro.2024.103013
%X DNA-binding proteins perform diverse functions, including regulating cellular growth and orchestrating chromatin architecture. Here, we present a protocol to discover proteins specifically interacting with a hexanucleotide repeat DNA, the expansion of which is known as the most frequent genetic cause of familial C9orf72 amyotrophic lateral sclerosis and frontotemporal dementia. We describe steps to fish out DNA-binding proteins recognizing double-stranded repeat DNAs using a SILAC (stable isotope labelling by amino acids in cell culture)-based approach and validate the results using electrophoretic mobility shift assay. For complete details on the use and execution of this protocol, please refer to Liu et al.1.