在成鱼中,神经发生发生在大脑的许多区域,包括小脑,新形成的细胞相对于脑细胞总数的比率比哺乳动物大几个数量级。我们的研究目的是比较芳香化酶B(AroB)的表达,谷氨酰胺合成酶(GS),和完整的幼年鲑鱼小脑中的胱氨酸-β-合酶(CBS),Oncorhynchusketa.为了确定确定AroB参与的动态,GS,和CBS在再生的细胞机制中,我们对这些分子标志物在长期原发性创伤性脑损伤(TBI)期间和反复急性小脑TBI后的表达进行了全面评估。因此,在完整的青少年中,AroB的弱或中等表达,GS,在四种细胞类型中检测到CBS,包括神经上皮类型的细胞,迁移,和分化的细胞(图形摘要,A).受伤后90天,在含有中度标记的AroB+的分子层中发现了局部高细胞区域,GS+,神经上皮型和较大的AroB+的CBS+细胞,GS+,和CBS细胞(可能类似于哺乳动物的反应性神经胶质);还观察到细胞迁移和新血管形成的模式。反复的TBI导致AroB+的数量,GS+,和CBS+细胞进一步增加;从所有细胞类型中记录到免疫标记的强度增加(图形摘要,C).因此,这项研究的结果为硬骨鱼的成年神经发生提供了更好的理解,这有望阐明哺乳动物中成年神经发生的再激活问题。
In adult fish, neurogenesis occurs in many areas of the brain, including the cerebellum, with the ratio of newly formed cells relative to the total number of brain cells being several orders of magnitude greater than in mammals. Our study aimed to compare the expressions of aromatase B (AroB), glutamine synthetase (GS), and cystathionine-beta-synthase (CBS) in the cerebellum of intact juvenile chum salmon, Oncorhynchus keta. To identify the dynamics that determine the involvement of AroB, GS, and CBS in the cellular mechanisms of regeneration, we performed a comprehensive assessment of the expressions of these molecular markers during a long-term primary traumatic brain injury (TBI) and after a repeated acute TBI to the cerebellum of O. keta juveniles. As a result, in intact juveniles, weak or moderate expressions of AroB, GS, and CBS were detected in four cell types, including cells of the neuroepithelial type, migrating, and differentiated cells (graphic abstract, A). At 90 days post injury, local hypercellular areas were found in the molecular layer containing moderately labeled AroB+, GS+, and CBS+ cells of the neuroepithelial type and larger AroB+, GS+, and CBS+ cells (possibly analogous to the reactive glia of mammals); patterns of cells migration and neovascularization were also observed. A repeated TBI caused the number of AroB+, GS+, and CBS+ cells to further increase; an increased intensity of immunolabeling was recorded from all cell types (graphic abstract, C). Thus, the results of this study provide a better understanding of adult neurogenesis in teleost fishes, which is expected to clarify the issue of the reactivation of adult neurogenesis in mammalian species.