黑色素瘤是最危险的皮肤癌,随着过去几年发病率的突然增长。它对化疗和放疗等传统治疗方法非常有抵抗力,但是这种癌症的治疗方法正在引起人们的关注。光动力疗法(PDT)被认为是治疗几种类型的皮肤癌的有效方式,并且可以提供治疗最具侵袭性的一种:黑色素瘤的可能性。在这项工作中,通过将培养的细胞暴露于5,10,15-三(五氟苯基)-20-(4-吡啶基)卟啉(PS1)及其二氢卟啉(PS2)和异杆菌二氢卟啉(PS3)相应衍生物和红色LED光(λ=660±20nm),来评估PDT对黑色素细胞系(B16F10细胞)的影响.使用MTT测定法测量细胞活力中的PDT效应。通过流式细胞术对细胞凋亡进行定量,并通过荧光显微镜确定光敏剂的亚细胞定位。此外,PS2产生超氧自由基的能力通过酪氨酸硝化定性评估。结果表明,PDT过程的效率取决于PS的结构及其产生单线态氧的能力。除此之外,光活化效率高度依赖于PS的细胞亚定位及其细胞摄取和单线态氧的产生。我们还发现,耐药细胞系B16F10具有独特的氯苷,异杆菌氯,或卟啉特异性抗性谱。此外,表明,高度荧光的二氢氯苷衍生物PS2也可以在成像诊断中考虑。
Melanoma is the most dangerous form of skin cancer, with an abrupt growth of its incidence over the last years. It is extremely resistant to traditional treatments such as chemotherapy and radiotherapy, but therapies for this cancer are gaining attention. Photodynamic therapy (PDT) is considered an effective modality to treat several types of skin cancers and can offer the possibility to treat one of the most aggressive ones: melanoma. In this work, the effect of PDT on a melanotic cell line (B16F10 cells) was assessed by exposing cultured cells to 5,10,15-tris(pentafluorophenyl)-20-(4-pyridyl)porphyrin (PS1) and to its
chlorin (PS2) and isobacteriochlorin (PS3) corresponding derivatives and red LED light (λ = 660 ± 20 nm). The PDT effect in the cells\' viability was measured using the MTT assay. The cell apoptosis was quantified by flow cytometry, and the subcellular localization of the photosensitizer was determined by fluorescence microscopy. In addition, the ability of PS2 to generate superoxide radicals was qualitatively assessed by tyrosine nitration. The results show that the efficiency of the PDT process is dependent on the structure of the PS and on their ability to produce singlet oxygen. Besides that, the photoactivation efficiency is highly dependent on the cellular sublocalization of the PS and on its cellular uptake and singlet oxygen production. We also found that the resistant cell line B16F10 has distinctive
chlorin, isobacteriochlorin, or porphyrin-specific resistance profiles. Furthermore, it is shown that the highly fluorescent
chlorin derivative PS2 can also be considered in imaging diagnostics.