为了发现具有生物效应的新自然资源,从in虫根中分离出的球形毛壳提取物的抗氧化和抗菌活性的化学研究。气相色谱-质谱(GC-MS)分析表明甲醇提取物中存在的主要化学成分(1,3-氧硫烷,1,3-环戊二烯,5-(1-甲基亚乙基),5,9-十六碳二烯酸,甲酯,癸烷),氯仿提取物(乙酸,二乙氧基-,乙酯,2,2-双(乙基磺酰基)丙烷,3-甲基-2-(2-氧代丙基)呋喃,和己烷提取物(3-己酮,4,4-二甲基,癸烷,2,6-二甲基癸烷,癸烷,2,4,6-三甲基,癸烷,2,4,6-三甲基,1-丁亚磺酰胺,1,1,2,2,3,3,4,4-九氟-N-甲基,癸烷)。在氯仿提取物中确定的总化合物(56.2%),(54.72%)在己烷提取物中,和(65%)在甲醇提取物中。使用二苯基吡啶酰肼基自由基(DPPH)进行抗氧化作用。结果表明,甲醇提取物具有最高的抗DPPH作用,IC50值为37.61±1.37μg/mL,然后是氯仿和己烷提取物,IC50值为40.82±3.60和45.20±2.54μg/mL,分别。对提取物的抗真菌活性进行了评估,以对抗包括尖孢镰刀菌在内的病原体真菌,雪莲,黄生枝孢菌,和菌核病.甲醇和氯仿提取物对所有测试病原体显示出最大的抑制作用,而己烷提取物显示最小的抑制作用。
To discover new natural resources with biological effects, the chemical investigation of antioxidant and antimicrobial activities of extract\'s
Chaetomium globosum isolated from roots of Urginea indica. Gas chromatography-mass spectrometry (GC-MS) analysis demonstrated the presence of the major chemical constituents present in the methanol extract (1,3-oxathiolane, 1,3-cyclopentadiene, 5-(1-methylethylidene), 5,9-hexadecadienoic acid, methyl ester, decane), chloroform extract (acetic acid, diethoxy-, ethyl ester, 2,2-bis(ethylsulfonyl)propane, 3-methyl-2-(2-oxopropyl) furan), and hexane extract (3-hexanone, 4,4-dimethyl, decane,2,6-dimethyldecane, decane, 2,4,6-trimethyl, decane, 2,4,6-trimethyl, 1-butanesulfinamide, 1,1,2,2,3,3,4,4,4-nonafluoro-N-methyl, decane). The total compound identified (56.2%) in chloroform extract, (54.72%) in hexane extract, and (65%) in methanol extract. The antioxidant effects were performed using diphenylpicrylhydrazyl radical (DPPH). The results showed that the methanol extract showed significantly the highest anti-DPPH with an IC50 value of 37.61 ± 1.37 μg/mL, followed by chloroform and hexane extracts with IC50 values of 40.82 ± 3.60 and 45.20 ± 2.54 μg/mL, respectively. The antifungal activity of extracts was evaluated against pathogens fungi including Fusarium oxysporum, Rosellinia necatrix, Cladosporium xanthochromaticum, and Sclerotinia sclerotiorum. Methanolic and chloroform extracts showed maximum inhibition against all test pathogens, while hexane extract showed minimum inhibition.