PDF(Pubmed)
Abstract：
Rice straw breakdown is sluggish, which makes agricultural waste management difficult, however pretreatment procedures and cellulolytic fungi can address this issue. Through ITS sequencing, Chaetomium globosum C1, Aspergillus sp. F2, and Ascomycota sp. SM2 were identified from diverse sources. Ascomycota sp. SM2 exhibited the highest carboxymethyl cellulase (CMCase) activity (0.86 IU/mL) and filter-paper cellulase (FPase) activity (1.054 FPU/mL), while Aspergillus sp. F2 showed the highest CMCase activity (0.185 IU/mL) after various pretreatments of rice straw. These fungi thrived across a wide pH range, with Ascomycota sp. SM2 from pH 4 to 9, Aspergillus sp. F2, and Chaetomium globosum C1 thriving in alkaline conditions (pH 9). FTIR spectroscopy revealed significant structural changes in rice straw after enzymatic hydrolysis and solid-state fermentation, indicating lignin, cellulose, and hemicellulose degradation. Soil amendments with pretreated rice straw, cow manure, biochar, and these fungi increased root growth and soil nutrient availability, even under severe salt stress (up to 9.3 dS/m). The study emphasizes the need for a better understanding of Ascomycota sp. degradation capabilities and proposes that using cellulolytic fungus and pretreatment rice straw into soil amendments could mitigate salt-related difficulties and improve nutrient availability in salty soils.
摘要：
稻草分解缓慢，这使得农业废物管理变得困难，然而预处理程序和纤维素分解真菌可以解决这个问题。通过ITS排序，球形毛壳菌C1，曲霉属。F2和子囊。SM2从不同来源鉴定。Ascomycotasp.SM2表现出最高的羧甲基纤维素酶（CMCase）活性（0.86IU/mL）和滤纸纤维素酶（FPase）活性（1.054FPU/mL），而曲霉属。在对稻草进行各种预处理后，F2显示出最高的CMCase活性（0.185IU/mL）。这些真菌在很宽的pH范围内繁殖，用Ascomycotasp.SM2从pH4到9，曲霉属。F2和球形毛霉C1在碱性条件（pH9）下蓬勃发展。FTIR光谱显示，酶水解和固态发酵后，稻草的结构发生了显着变化，指示木质素，纤维素,和半纤维素降解。预处理稻草的土壤改良剂，牛粪,生物炭,这些真菌增加了根系生长和土壤养分利用率，即使在严重的盐胁迫下（高达9.3dS/m）。该研究强调需要更好地了解Ascomycotasp。降解能力，并提出使用纤维素分解真菌和将稻草预处理到土壤改良剂中可以减轻与盐有关的困难并提高盐渍土壤中的养分利用率。
