简介:生物聚合物,比如普鲁兰,一种天然的外多糖,它们的纳米复合材料通常用于食品中,Pharmaceutical,和医疗行业由于其独特的物理和化学性质。方法:普鲁兰杆菌ATCC201253菌株合成普鲁兰多糖。制备了基于生物合成普鲁兰的纳米复合膜,并负载了由镰刀菌菌株JTW1合成的不同浓度的银纳米颗粒(AgNPs)。AgNPs通过透射电子显微镜表征,Zeta电位测量,和傅里叶变换红外光谱。反过来,生产的薄膜进行了物理化学分析,如测角法,紫外线屏蔽能力,衰减全反射-傅里叶变换红外光谱,扫描电子显微镜,和X射线光电子能谱,并对其机械性能和降解性能进行了评估。纳米颗粒和纳米复合膜对食源性病原体和参考病原体的抗菌试验,包括单核细胞增生李斯特菌,沙门氏菌婴儿,肠沙门氏菌,大肠杆菌,金黄色葡萄球菌,铜绿假单胞菌,和肺炎克雷伯菌,使用标准方法进行。结果:AgNPs小(平均15.1nm),球形,并表现出良好的稳定性,涂有蛋白质生物分子。以更高的浓度用作支链淀粉膜的添加剂时,它们导致UV-B和UV-A光的亲水性和透光率降低。此外,所产生的膜表现出光滑的表面。因此,可以得出结论,与对照膜相比,添加生物AgNPs没有改变膜的形态和质地。纳米颗粒和纳米复合膜对食源性细菌和参考细菌均具有显着的抗菌活性。所制备的膜对单核细胞增生李斯特菌的活性最高。讨论:获得的结果表明,由生物合成的普鲁兰多糖和AgNP制备的新型纳米复合膜可考虑用于开发医疗产品和食品包装。此外,这是关于支链淀粉基纳米复合材料与真菌AgNPs用于此类应用的第一份报告。
Introduction: Biopolymers, such as pullulan, a natural exopolysaccharide from Aureobasidium pullulans, and their nanocomposites are commonly used in the food, pharmaceutical, and medical industries due to their unique physical and chemical properties. Methods: Pullulan was synthesized by the A. pullulans ATCC 201253 strain. Nanocomposite films based on biosynthesized pullulan were prepared and loaded with different concentrations of silver nanoparticles (AgNPs) synthesized by the Fusarium culmorum strain JTW1. AgNPs were characterized by transmission electron microscopy, Zeta potential measurements, and Fourier-transform infrared spectroscopy. In turn, the produced films were subjected to physico-chemical analyses such as goniometry, UV shielding capacity, attenuated total reflection-Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy, and their mechanical and degradation properties were assessed. The antibacterial assays of the nanoparticles and the nanocomposite films against both food-borne and reference pathogens, including Listeria monocytogenes, Salmonella infantis, Salmonella enterica, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, were performed using standard methods. Results: AgNPs were small (mean 15.1 nm), spherical, and displayed good stability, being coated with protein biomolecules. When used in higher concentrations as an additive to pullulan films, they resulted in reduced hydrophilicity and light transmission for both UV-B and UV-A lights. Moreover, the produced films exhibited a smooth surface. Therefore, it can be concluded that the addition of biogenic AgNPs did not change the morphology and texture of the films compared to the control film. The nanoparticles and nanocomposite films demonstrated remarkable antibacterial activity against both food-borne and reference bacteria. The highest activity of the prepared films was observed against L. monocytogenes. Discussion: The obtained results suggest that the novel nanocomposite films prepared from biosynthesized pullulan and AgNPs can be considered for use in the development of medical products and food packaging. Moreover, this is the first report on pullulan-based nanocomposites with mycogenic AgNPs for such applications.