PDF(Pubmed)
Abstract：
Post-harvest decay of fresh table grapes causes considerable annual production losses. The main fungal agents of decay both in pre- and post-harvest are B. cinerea, Penicillium spp., Aspergillus spp., Alternaria spp., and Cladosporium spp. To date, the use of agrochemicals and SO2 are the main methods to control grape molds in pre- and postharvest, respectively. Significant improvements, however, have already been made in to apply innovative and more environmentally sustainable control strategies, such as Biological Control Agents (BCAs), which can reduce disease severity in both pre- and post-harvest. In this study, 31 new non-Saccharomyces yeast strains, isolated from berries of native Apulian table grape genotypes, were tested for their in vivo effectiveness against grey mold of table grapes, resulting in two St. bacillaris (\'N22_I1\' and \'S13_I3\'), one S. diversa (\'N22_I3\'), one A. pullulans (\'OLB_9.1_VL\') and one H. uvarum (\'OLB_9.1_BR\') yeast strains that were marked as efficient and good BCAs. Their mechanisms of action were characterized through in vitro assays, and additional characteristics were evaluated to assess the economic feasibility and viability for future technological employment. Their effectiveness was tested by reducing the working concentration, their antagonistic effect on a wide range of fungal pathogens, their ability to survive in formulations with long shelf life, and their safety to human health.
摘要：
新鲜鲜食葡萄的收获后腐烂会导致相当大的年度生产损失。收获前和收获后腐烂的主要真菌是灰霉病菌，青霉属。,曲霉属。,链格孢菌属。,和枝孢菌属。迄今为止,农用化学品和SO2的使用是在采前和采后控制葡萄霉菌的主要方法，分别。重大改进,然而,已经制定了应用创新和更环境可持续的控制策略，如生物防治剂(BCA)，可以降低收获前和收获后的疾病严重程度。在这项研究中,31个新的非酵母菌株，从本地Apulian食用葡萄基因型的浆果中分离出来，测试了它们在体内对抗葡萄灰霉病的有效性，导致两个圣芽孢杆菌(\'N22_I1\'和\'S13_I3\')，一个S.diversa(\'N22_I3\')，1株普鲁兰(\'OLB_9.1_VL\')和1株H.uvarum(\'OLB_9.1_BR\')酵母菌株被标记为有效和良好的BCA。它们的作用机制通过体外试验进行了表征，和其他特征进行了评估，以评估未来技术就业的经济可行性和可行性。通过降低工作浓度来测试它们的有效性，它们对各种真菌病原体的拮抗作用，它们在具有长保质期的制剂中存活的能力，以及他们对人类健康的安全。
