PDF(Pubmed)
Abstract：
Different preventive strategies are needed to minimize the intake risks of mycotoxins, including zearalenone (ZEN). The aim of this study was to determine the ZEN adsorption ability of an autolyzed biomass preparation of polymorphic yeast Aureobasidium pullulans A.p.-3. The evaluation of the antitoxic properties of the preparation was also performed in relation to Saccharomyces cerevisiae yeast (ATCC 2366, ATCC 7090 and ATCC 9763) used as a model cell exposed to a toxic ZEN dose. The preparation at a dose of 5 mg/mL showed the adsorption of ZEN present in model systems at concentrations between 1 μg/mL to 100 μg/mL. The highest degree of adsorption was established for ZEN concentrations of 1 μg/mL and 5 μg/mL, becoming limited at higher doses of the toxin. Based on the Langmuir model of adsorption isotherms, the predicted maximum ZEN adsorption was approx. 190 µg/mL, regardless of pH. The growth of three strains of S. cerevisiae yeast cells in the medium with ZEN at concentrations within the range of 1.56 μg/mL-100 μg/mL was analyzed to determine the minimum inhibitory concentration. The growth of all tested strains was especially limited by high doses of ZEN, i.e., 50 and 100 μg/mL. The protective effect of the tested preparation was noted in relation to yeast cells exposed to toxic 100 μg/mL ZEN doses. The highest yeast cell growth (app. 36% percentage) was noted for a S. cerevisiae ATCC 9763 strain compared to the medium with ZEN but without preparation. More detailed tests determining the antitoxic mechanisms of the A. pullulans preparation are planned in the future, including cell culture bioassays and animal digestive tract models.
摘要：
需要不同的预防策略来最大程度地减少霉菌毒素的摄入风险，包括玉米赤霉烯酮（ZEN）。本研究的目的是确定多态酵母出芽金霉A.p.-3的自溶生物质制剂的ZEN吸附能力。还对用作暴露于毒性ZEN剂量的模型细胞的酿酒酵母(ATCC2366、ATCC7090和ATCC9763)进行了制剂的抗毒性质的评估。剂量为5mg/mL的制剂显示出在1μg/mL至100μg/mL的浓度下存在于模型系统中的ZEN的吸附。ZEN浓度为1μg/mL和5μg/mL时，吸附度最高。在较高剂量的毒素下变得有限。基于吸附等温线的Langmuir模型,预测的最大ZEN吸附量约为。190微克/毫升，不管pH值。分析三种酿酒酵母细胞菌株在含有浓度在1.56μg/mL-100μg/mL范围内的ZEN的培养基中的生长以确定最小抑制浓度。所有测试菌株的生长尤其受到高剂量ZEN的限制，即，50和100μg/mL。相对于暴露于有毒的100μg/mLZEN剂量的酵母细胞,注意到测试制剂的保护作用。最高的酵母细胞生长（应用。与含ZEN但未制备的培养基相比，酿酒酵母ATCC9763菌株的百分比为36％）。未来计划进行更详细的测试，以确定支链淀粉制剂的抗毒机制。包括细胞培养生物测定和动物消化道模型。
