早疫病(EB),由SolaniAlternaria引起,是番茄生产中的一个严重问题。植物促生长根瘤菌促进植物生长,抑制植物病害。本研究探讨了根瘤菌和姜粉提取物(GPE)对番茄EB病的协同作用,单独和组合。根据形态学特征,将有症状的番茄植物中的六种真菌分离株鉴定为A.solani,即水平间隔(6.96至7.93µm),垂直间隔(1.50至2.22µm),分生孢子长度(174.2至187.6µm),分生孢子宽度(14.09至16.52µm),喙长度(93.06至102.26µm),和孢子形成。从番茄根际土壤中回收的23种细菌中,有5种对番茄幼苗无致病性,并且彼此兼容,与GPE兼容。在单独测试的五个分离株中,三个分离株(St-149D,Hyd-13Z,和Gb-T23)显示最大抑制(56.3%,48.3%,分别为42.0%)对抗索兰尼的菌丝体生长。在组合中,与未处理的对照相比,St-149D+GPE具有最高的菌丝体生长抑制(76.9%)。分子特征为恶臭假单胞菌的细菌菌株,枯草芽孢杆菌,和蜡状芽孢杆菌,并通过种子细菌进行盆栽试验以控制疾病。用细菌聚生体+GPE处理的种子具有最高的疾病抑制百分比(78.1%),其次是St-149D+GPE(72.2%)和Hyd-13Z+GPE(67.5%)。在细菌聚生体GPE(95.0±2.04),然后是St-149DGPE(92.5±1.44)和Hyd-13ZGPE(90.0±2.04),比对照(73.8±2.39)和化学对照(90.0±2)获得了最大的种子萌发。姜粉提取物还诱导防御相关酶的激活(TPC,PO,PPO,PAL,和CAT)在番茄植物中的活性。这些在测试细菌接种剂对抗番茄作物中的solani感染中非常重要。
Early blight (EB), caused by Alternaria solani, is a serious problem in tomato production. Plant growth-promoting rhizobacteria promote plant growth and inhibit plant disease. The present study explored the bio-efficacy of synergistic effect of rhizobacterial isolates and ginger powder extract (GPE) against tomato EB disease, singly and in combination. Six fungal isolates from symptomatic tomato plants were identified as A. solani on the basis of morphological features i.e., horizontal septation (6.96 to 7.93 µm), vertical septation (1.50 to 2.22 µm), conidia length (174.2 to 187.6 µm), conidial width (14.09 to 16.52 µm), beak length (93.06 to 102.26 µm), and sporulation. Five of the twenty-three bacterial isolates recovered from tomato rhizosphere soil were nonpathogenic to tomato seedlings and were compatible with each other and with GPE. Out of five isolates tested individually, three isolates (St-149D, Hyd-13Z, and Gb-T23) showed maximum inhibition (56.3%, 48.3%, and 42.0% respectively) against mycelial growth of A. solani. Among combinations, St-149D + GPE had the highest mycelial growth inhibition (76.9%) over the untreated control. Bacterial strains molecularly characterized as Pseudomonas putida, Bacillus subtilis, and Bacillus cereus and were further tested in pot trials through seed bacterization for disease control. Seeds treated with bacterial consortia + GPE had the highest disease suppression percentage (78.1%), followed by St-149D + GPE (72.2%) and Hyd-13Z + GPE (67.5%). Maximum seed germination was obtained in the bacterial consortia + GPE (95.0 ± 2.04) followed by St-149D + GPE (92.5 ± 1.44) and Hyd-13Z + GPE (90.0 ± 2.04) over control (73.8 ± 2.39) and chemical control as standard treatment (90.0 ± 2). Ginger powder extracts also induce the activation of defence-related enzymes (TPC, PO, PPO, PAL, and CAT) activity in tomato plants. These were highly significant in the testing bacterial inoculants against A. solani infection in tomato crops.