近年来,由于通过不同的机制为植物提供了促进生长的益处,因此在农业中使用与植物生长促进细菌(PGPB)一起配制的商业细菌接种剂已显示出显着的重要性。然而,接种剂中细菌细胞的存活和活力在使用过程中受到影响,并可能降低其有效性。解决生存能力问题的生理适应策略引起了人们的关注。这篇综述旨在概述选择亚致死应激策略以提高细菌接种剂有效性的研究。搜索是在2021年11月使用WebofScience进行的,Scopus,PubMed,和Proquest数据库。关键词\"固氮菌\",“促进植物生长的根瘤菌”,\"偶氮螺旋菌\",\"假单胞菌\",“根瘤菌”,“应力预处理”,\"适应\",“代谢生理适应”,“蜂窝适应”,“增加生存”,搜索中使用了“保护剂”和“保护策略”。共发现2573种出版物,选择了34项研究进行更深入的研究。根据研究分析,确定了与亚致死应激相关的差距和潜在应用。最常用的策略包括渗透,热,氧化,营养压力,原代细胞对胁迫的反应机制是渗透压的积累,植物激素,和胞外多糖(EPS)。在亚致死压力下,冻干后接种剂存活率呈正增量,干燥,和长期存储过程。亚致死胁迫后,接种体-植物相互作用的有效性也有正增量,改善植物发育,疾病控制,与无吸引力的接种剂相比,对环境压力的耐受性。
The use of commercial bacterial inoculants formulated with plant-growth promoting bacteria (PGPB) in agriculture has shown significant prominence in recent years due to growth-promotion benefits provided to plants through different mechanisms. However, the survival and viability of bacterial cells in inoculants are affected during use and may decrease their effectiveness. Physiological adaptation strategies have attracted attention to solve the viability problem. This
review aims to provide an overview of research on selecting sublethal stress strategies to increase the effectiveness of bacterial inoculants. The searches were performed in November 2021 using Web of Science, Scopus, PubMed, and Proquest databases. The keywords \"nitrogen-fixing bacteria\", \"plant growth-promoting rhizobacteria\", \"azospirillum\", \"pseudomonas\", \"rhizobium\", \"stress pre-conditioning\", \"adaptation\", \"metabolic physiological adaptation\", \"cellular adaptation\", \"increasing survival\", \"protective agent\" and \"protective strategy\" were used in the searches. A total of 2573 publications were found, and 34 studies were selected for a deeper study of the subject. Based on the studies analysis, gaps and potential applications related to sublethal stress were identified. The most used strategies included osmotic, thermal, oxidative, and nutritional stress, and the primary cell response mechanism to stress was the accumulation of osmolytes, phytohormones, and exopolysaccharides (EPS). Under sublethal stress, the inoculant survival showed positive increments after lyophilization, desiccation, and long-term storage processes. The effectiveness of inoculant-plants interaction also had positive increments after sublethal stress, improving plant development, disease control, and tolerance to environmental stresses compared to unappealed inoculants.