弓形虫(T.gondii)是一种人畜共患寄生虫,可在人类和动物等中间宿主中引起严重的病理。吃未煮熟或生肉是弓形虫感染的最重要途径。绵羊是全世界重要的肉类来源,它们也容易感染弓形虫。羊肉感染弓形虫对消费者的食品安全构成严重威胁。目前,研究主要集中在牲畜中弓形虫的感染率;然而,尚未对该寄生虫在动物不同肌肉和内脏组织中的分布差异进行系统研究。在这项研究中,研究了弓形虫在自然感染的小尾寒羊中的分布差异。通过实时荧光定量PCR(RT-qPCR)扩增寄生虫的B1基因,我们发现弓形虫的寄生虫负担在绵羊的不同部位不同,在内脏的心脏和肌肉的外部脊观察到最高的负担。在我们的研究中,相对表达从高到低排序如下:心脏,脾,脾外部山脊,里脊肉,肺,肝脏,肾,脖子肉,前肢,黄瓜条,后腿,羊肚,还有羊排.本研究为羊肉制品食品安全监测提供了重要指导。
Toxoplasma gondii (T. gondii) is a zoonotic parasite that can cause serious pathology in intermediate hosts such as humans and animals. Eating undercooked or raw meat is the most important route of infection by T. gondii. Sheep are an important source of meat worldwide, and they are also susceptible to T. gondii. Mutton infected with T. gondii poses a serious threat to the food safety of consumers. At present, studies have mainly focused on the infection ratio of T. gondii in livestock; however, systematic studies have not been performed on differences in the distribution of this parasite in different muscle and
viscera tissues of animals. In this study, the differences in the distribution of T. gondii in naturally infected Small-tailed Han sheep was studied. By amplifying the B1 gene of the parasite via real-time fluorescence quantification PCR (RT‒qPCR), we found that the parasite burden of T. gondii differed among different parts of the sheep, with the highest burden observed in the heart among the
viscera and the external ridge among the muscle. The relative expression was ranked from high to low in our study as follows: heart, spleen, external ridge, tenderloin, lung, liver, kidney, neck meat, forelegs, cucumber strips, hind leg, lamb belly, and lamb chops. This study provided important guidance for monitoring the food safety of mutton products.