■作为保守的信号通路,丝裂原活化蛋白激酶(MAPK)级联调节细胞对非生物胁迫的信号传导。高温可能导致经济产量的显著降低。然而,对高温下StMAPKK家族基因表达模式的研究有限,并且缺乏关于它们在支持马铃薯植物生长中的作用的实验验证。
■为了触发热应激反应,马铃薯植物在35°C生长。通过qRT-PCR分析了StMAPKK家族基因在马铃薯植株中的表达模式。开发了具有StMAPKK5功能丧失和功能获得的植物。马铃薯的生长和形态特征通过测量株高,干重,新鲜的重量。StMAPKK5的抗氧化能力由抗氧化酶活性和H2O2含量表示。细胞膜的完整性和通透性由相对电导率(REC)提出,丙二醛和脯氨酸含量。接下来测定光合能力。Further,检测了热应激响应基因和抗氧化酶基因的mRNA表达。
■在对热应力的反应中,改变了StMAPKK家族基因的表达谱。StMAPKK5蛋白位于细胞核,细胞质和细胞膜,在热胁迫条件下控制马铃薯植株的身高和体重方面发挥作用。StMAPKK5过表达促进光合作用并维持细胞膜完整性,在热胁迫下抑制蒸腾作用和气孔导度。StMAPKK5的过表达引发了马铃薯植物对热胁迫的生化防御,调节H2O2、MDA和脯氨酸的水平,以及CAT的抗氧化活性,SOD和POD。StMAPKK5的过表达引发了马铃薯植物对热胁迫的遗传反应,影响热应激响应基因和编码抗氧化酶的基因。
■StMAPKK5可以提高马铃薯植株对热胁迫引起的损害的抗逆性,为工程马铃薯提供了一种有希望的方法,该方法对具有挑战性的热应激条件具有增强的适应性。
UNASSIGNED: As a conserved signaling pathway, mitogen-activated protein kinase (MAPK) cascade regulates cellular signaling in response to abiotic stress. High temperature may contribute to a significant decrease in economic yield. However, research into the expression patterns of StMAPKK family genes under high temperature is limited and lacks experimental validation regarding their role in supporting potato plant growth.
UNASSIGNED: To trigger heat stress responses, potato plants were grown at 35°C. qRT-PCR was conducted to analyze the expression pattern of StMAPKK family genes in potato plants. Plant with StMAPKK5 loss-of-function and gain-of-function were developed. Potato growth and morphological features were assessed through measures of plant height, dry weight, and fresh weight. The antioxidant ability of StMAPKK5 was indicated by antioxidant enzyme activity and H2O2 content. Cell membrane integrity and permeability were suggested by relative electrical conductivity (REC), and contents of MDA and proline. Photosynthetic capacity was next determined. Further, mRNA expression of heat stress-responsive genes and antioxidant enzyme genes was examined.
UNASSIGNED: In reaction to heat stress, the expression profiles of StMAPKK family genes were changed. The StMAPKK5 protein is located to the nucleus, cytoplasm and cytomembrane, playing a role in controlling the height and weight of potato plants under heat stress conditions. StMAPKK5 over-expression promoted photosynthesis and maintained cell membrane integrity, while inhibited
transpiration and stomatal conductance under heat stress. Overexpression of StMAPKK5 triggered biochemical defenses in potato plant against heat stress, modulating the levels of H2O2, MDA and proline, as well as the antioxidant activities of CAT, SOD and POD. Overexpression of StMAPKK5 elicited genetic responses in potato plants to heat stress, affecting heat stress-responsive genes and genes encoding antioxidant enzymes.
UNASSIGNED: StMAPKK5 can improve the resilience of potato plants to heat stress-induced damage, offering a promising approach for engineering potatoes with enhanced adaptability to challenging heat stress conditions.