这项研究的主要目的是研究硅(Si;2mM)对钒(V;40mg)胁迫下番茄幼苗生长的缓解作用。V胁迫导致叶片中更高的V吸收,和提高叶花青素的浓度,H2O2,O2•-,MDA,但是植物生物量的减少,根架构系统,叶色素含量,矿物元素,和Fv/Fm(PSII最大效率)。硅的应用增加了关键抗氧化剂分子如AsA和GSH的浓度,以及包含APX的关键抗氧化酶的作用,GR,DHAR,MDHAR重要的是,这些抗氧化酶基因的上调可显着减轻氧化损伤。此外,Si的应用增强了次生代谢产物的积累及其相关基因的表达,这些次生代谢产物可能限制了H2O2的过度积累。此外,Si通过增强Gly酶活性来拯救番茄植株免受MG的破坏作用。结果证实,向植物喷洒Si可能会减少植物对V的可及性,随着抗V应力能力的提高。
The primary goal of this research is to investigate the mitigating effect of silicon (Si; 2 mM) on the growth of tomato seedlings under vanadium (V; 40 mg) stress. V stress caused higher V uptake in leaf, and enhanced concentration of leaf anthocyanin, H2O2, O2•-, and MDA, but a decreased in plant biomass, root architecture system, leaf pigments content, mineral elements, and Fv/Fm (PSII maximum efficiency). Si application increased the concentrations of crucial antioxidant molecules such as AsA and GSH, as well as the action of key antioxidant enzymes comprising APX, GR, DHAR, and MDHAR. Importantly, oxidative damage was remarkably alleviated by upregulation of these antioxidant enzymes genes. Moreover, Si application enhanced the accumulation of secondary metabolites as well as the expression their related-genes, and these secondary metabolites may restricted the excessive accumulation of H2O2. In addition, Si rescued tomato plants against the damaging effects of MG by boosting the Gly enzymes activity. The results confirmed that spraying Si to plants might diminish the V accessibility to plants, along with promotion of V stress resistance.