犬圆环病毒(CanineCV)是最近鉴定的圆环病毒科成员。自2011年被发现以来,CanineCV已在全球不同国家被检测到,感染家畜和野生犬科动物。该病毒可能与胃肠道和呼吸道疾病有关。2016年,广西西南地区报告了CanineCV,中国。然而,其在西南地区其他省份的患病率仍然未知。本研究收集了四川地区家犬血清样本208份,中国将于2022年调查犬的患病率。在这些样本中,26的CanineCV检测呈阳性,结果阳性率为12.5%。此外,对12个菌株进行了测序,其中9个具有2,063个核苷酸(nt)的序列长度,其他三个中的2个长度为2,062nt,另一个为2,064nt。值得注意的是,鉴定出一个移码突变,导致截短的ORF1,并在复制蛋白(Rep)的末端出现由13个氨基酸组成的新序列。这种突变可能会影响病毒的复制周期。系统发育和进化分析表明,分离株属于CanineCV-3基因型,流行于中国东南部和西南地区,以及在邻国与其他相同基因型的菌株。总的来说,这项流行病学调查扩大了我们对中国西南地区犬科动物的遗传多样性的理解,并提供了对病毒进化的见解。
Canine circovirus (CanineCV) is a recently identified member of the Circoviridae family. Since its discovery in 2011, CanineCV has been detected in different countries worldwide, infecting both domestic and wild canids. The virus is potentially associated with gastrointestinal and respiratory illnesses. In 2016, CanineCV was reported in the southwestern region of Guangxi,
China. However, its prevalence in other provinces in the Southwest region remained unknown. This study collected a total of 208 serum samples from domestic dogs in Sichuan,
China in 2022 to investigate the prevalence of CanineCV. Among these samples, 26 tested positive for CanineCV, resulting in a positivity rate of 12.5%. Additionally, 12 strains were sequenced, 9 of which had a sequence length of 2,063 nucleotides (nt), 2 of the other threes had a length of 2,062 nts and another was 2,064 nt. Notably, a frameshift mutation was identified, resulting in a truncated ORF1 and the occurrence of a novel sequence comprised of 13 amino acids at the end of the replicate protein (Rep). This mutation could affect the replication cycle of the virus. Phylogenetic and evolutionary analyses revealed that the isolates belonged to the CanineCV-3 genotype and were prevalent in the Southeast and the Southwest regions of
China, as well as in the neighboring countries alongside other strains of the same genotype. Collectively, this epidemiological investigation widens our understanding of the genetic diversity of CanineCV in Southwest
China and provides insights into viral evolution.