PDF(Pubmed)
Abstract：
Pseudorabies have caused enormous economic losses in China\'s pig industry and have recurred on many large pig farms since late 2011. The disease is caused by highly pathogenic, antigenic variant pseudorabies virus (vPRV) strains. Our laboratory isolated a pseudorabies virus in 2015 and named it XJ5. The pathogenic ability of this mutant strain was much stronger than that of the original isolate. After we sequenced its whole genome (GenBank accession number: OP512542), we found that its overall structure was not greatly changed compared with that of the previous strain Ea (KX423960.1). The whole genome alignment showed that XJ5 had a strong genetic relationship with the strains isolated in China after 2012 reported in GenBank. Based on the isolation time of XJ5 and the mutation and recombination analysis of programs, we found that the whole genome homology of XJ5 and other strains with Chinese isolates was greater than 95%, while the homology with strains outside Asia was less than 94%, which indicated that there may be some recombination and mutation patterns. We found that virulent PRV isolates emerged successively in China in 2011 and formed two different evolutionary clades from foreign isolates. At the same time, this may be due to improper immunization and the presence of wild strains in the field, and recent reports have confirmed that Bartha vaccine strains recombine with wild strains to obtain new pathogenic strains. We performed genetic evolution analysis of XJ5 isolated and sequenced in our laboratory to trace its possible mutations and recombination. We found that XJ5 may be the result of natural mutation of a virus in a branch of mutant strains widely existing in China.
摘要：
猪伪狂犬病给我国养猪业造成了巨大的经济损失,自2011年底以来在许多大型养猪场反复发作。这种疾病是由高致病性引起的，抗原变异伪狂犬病病毒（vPRV）株。我们的实验室在2015年分离出了一种伪狂犬病病毒，并将其命名为XJ5。该突变株的致病能力比原始分离株强得多。在我们对其整个基因组进行测序后（GenBank登录号：OP512542），我们发现它的整体结构与以前的菌株Ea（KX423960.1）相比没有太大变化。全基因组比对显示,2012年在GenBank报道后,XJ5与中国分离的菌株具有较强的亲缘关系。基于XJ5的分离时间和突变重组分析的程序,我们发现XJ5和其他菌株与中国分离株的全基因组同源性大于95%,与亚洲以外的菌株的同源性低于94%，这表明可能存在一些重组和突变模式。我们发现2011年在中国相继出现了强毒株PRV，并与国外分离株形成了两个不同的进化枝。同时,这可能是由于免疫不当和野外野生菌株的存在，最近的报道证实，Bartha疫苗株与野生株重组获得新的致病株。我们在实验室中对分离和测序的XJ5进行了遗传进化分析，以追踪其可能的突变和重组。我们发现XJ5可能是在中国广泛存在的突变株分支中病毒自然突变的结果。
