虹鳟鱼是世界范围内广泛养殖的经济冷水鱼,但是传染性造血器官坏死病毒(IHNV)的流行给水产养殖业带来了严重的风险,造成高死亡率和巨大的经济损失。在这项研究中,评估了不同浓度(0、10、20和30g/kg)的中药混合物(CHMM)对虹鳟鱼对IHNV感染的免疫反应和抵抗力的影响。结果表明,CHMM显著升高(P<0.05)T-SOD,CAT,AST,ALT,ACP,和AKP活性和MDA含量降低。NF-κB,TNF-α,IFN-β,IL-1β,在所有CHMM中JAK1、HSP70和HSP90表达均显著上调(P<0.05),SOCS2表达下调(P<0.05)。感染IHNV后,用不同数量的CHMM喂养虹鳟鱼导致T-SOD明显增加(P<0.05),ACP,和AKP活性明显下降(P<0.05)MDA含量、AST和ALT活性。TNF-α,IFN-β,IL-1β,HSP70和HSP90在所有CHMM中的表达均显著上调(P<0.05)。而JAK1和SOCS2的表达下调。20g/kg剂量的IHNVG蛋白基因的表达水平明显低于其他CHMM喂养组。本研究为促进CHMM作为提高虹鳟鱼抗病毒免疫力的免疫刺激剂提供了坚实的科学依据。
Rainbow trout is a widely farmed economical cold-water fish worldwide, but the prevalence of infectious hematopoietic necrosis virus (IHNV) presents a severe risk to the aquaculture industry, resulting in high mortality and huge economic losses. In this study, the impacts of different concentrations (0, 10, 20, and 30 g/kg) of Chinese herbal medicine mixture (CHMM) on the immune response and resistance of rainbow trout to IHNV infection were evaluated. The results show that CHMM noticeably increased (P < 0.05) T-SOD, CAT, AST, ALT, ACP, and AKP activities and decreased MDA content. NF-κB, TNF-α, IFN-β, IL-1β, JAK1, HSP70, and HSP90 expressions were significantly upregulated (P < 0.05) in all CHMMs, while SOCS2 expression was downregulated (P < 0.05). Following infection with IHNV, feeding rainbow trout with varying amounts of CHMM resulted in noticeably increased (P < 0.05) T-SOD, ACP, and AKP activities and significantly decreased (P < 0.05) MDA content and AST and ALT activities. TNF-α, IFN-β, IL-1β, HSP70, and HSP90 expressions were significantly upregulated (P < 0.05) in all CHMMs, while the expressions of JAK1 and SOCS2 were downregulated. The expression level of the IHNV G protein gene at a dosage of 20 g/kg was notably lower than that of the other CHMM feeding groups. This study provides a solid scientific basis for promoting CHMM as an immunostimulant for boosting antiviral immunity in rainbow trout.