进行了综合分析,以探索在德国的土塘虹鳟鱼(Oncorhynchusmykiss)水产养殖中引起异味的气味分子。在这部分研究中,使用溶剂辅助风味蒸发(SAFE)提取水气味;然后,通过一维和二维高分辨率气相色谱结合嗅觉测定法和质谱,使用两个极性不同的柱(DB-FFAP和DB-5)分析提取物。溶剂提取物样品的香气提取物稀释分析(AEDA)揭示了54种气味,其中47人被确认身份。在这项研究中,在德国土塘虹鳟鱼养殖水中首次描述了一系列化合物,包括,在其他人中,4-羟基-2,5-二甲基-3(2H)-呋喃酮(呋喃醇),香兰素,(E)-4,5-环氧-(E)-2-癸酸,4-乙基辛酸,3-甲基吲哚(skatole),5α-雄激素-16-en-3-酮(雄烯酮),和2-(2-丁氧基乙氧基)乙醇。此外,感官实验表明,(E)-4,5-环氧-(E)-2-癸酸,(E,E)-2,4-八二烯醛,1-octen-3-one是金属的主要贡献者,黄瓜,和样品的蘑菇味。
Comprehensive analyses were accomplished to explore the odorous molecules responsible for off-odour development in earthen-ponds rainbow trout (Oncorhynchus mykiss) aquaculture farming in Germany. In this part of the study, water odorants were extracted using solvent-assisted flavour evaporation (SAFE); then, extracts were analysed by one- and two- dimensional high resolution gas chromatography coupled with olfactometry and mass spectrometry using two columns with different polarity (DB-FFAP and DB-5). Aroma extract dilution analysis (AEDA) of the solvent extract samples revealed 54 odorants, and 47 of them were identified. In this study, a series of compounds is described for the first time in German earthen-ponds rainbow trout aquaculture water including, amongst others, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (furaneol), vanillin, (E)-4,5-epoxy-(E)-2-decenal, 4-ethyloctanoic acid, 3-methylindole (skatole), 5α-androst-16-en-3-one (androstenone), and 2-(2-butoxyethoxy) ethanol. Moreover, the sensory experiment indicated that (E)-4,5-epoxy-(E)-2-decenal, (E,E)-2,4-octadienal, and 1-octen-3-one are the main contributors to the metallic, cucumber, and mushroom notes of the samples.