Abstract：
In this study, the impact of three unsaturated fatty acids (Oleic acid: OA, Eicosapentaenoic acid: EPA, Docosahexaenoic acid: DHA) on the oxidation and structure of rainbow trout myofibrillar protein (MP) was explored. The findings revealed a notable increase in carbonyl content (P < 0.05) and a significant decrease in total sulfhydryl content (P < 0.05) of MP with the concentration increase of the three unsaturated fatty acids. Endogenous fluorescence spectroscopy and surface hydrophobicity analyses showed that unsaturated fatty acids can cause unfolding and exposure of hydrophobic groups in MP. In addition, SDS-PAGE showed that disulfide bonds were associated with MP cross-linking and aggregate size induced by unsaturated fatty acids. Overall, three unsaturated fatty acid treatments facilitated the oxidation of myofibrillar proteins, and the extent of protein oxidation was closely associated with the concentration of unsaturated fatty acids.
摘要：
在这项研究中,三种不饱和脂肪酸(油酸：OA，二十碳五烯酸：EPA，二十二碳六烯酸：DHA）对虹鳟鱼肌原纤维卵白（MP）的氧化和构造停止了摸索。结果表明，随着三种不饱和脂肪酸浓度的增加，MP的羰基含量显着增加（P<0.05），总巯基含量显着降低（P<0.05）。内源性荧光光谱和表面疏水性分析表明，不饱和脂肪酸可引起MP中疏水基团的解折叠和暴露。此外,SDS-PAGE显示二硫键与不饱和脂肪酸诱导的MP交联和聚集体大小有关。总的来说,三种不饱和脂肪酸处理促进了肌原纤维蛋白的氧化，蛋白质氧化程度与不饱和脂肪酸浓度密切相关。
