背景:本研究旨在通过不同的技术通过暂时的腹部闭合来建立开放腹部(OA)的动物模型。
方法:成年雄性Sprague-Dawley大鼠随机分为三组:A组(OA单用聚丙烯网片);B组(OA单用聚丙烯网片加贴片);C组(OA单用聚丙烯网片加贴片)。生命体征,病理生理变化,术后7天密切监测大鼠的存活率。进行腹部X射线和组织病理学检查以评估腹部器官变化和伤口愈合。
结果:结果显示三组间死亡率无显著差异(p>0.05)。然而,B组大鼠总体状况优越,更清洁的伤口,与其他组相比,伤口愈合率更高(p<0.05)。腹部X线片显示各组均有不同程度的远端肠梗阻。组织病理学检查显示纤维增生,炎性细胞浸润,新生血管形成,各组胶原沉积。B组肉芽组织生成增强,新生血管形成,和胶原沉积与其他组相比(p<0.05)。
结论:聚丙烯网结合贴剂是建立OA动物模型的最合适方法。该模型成功复制了OA术后患者的病理和生理变化,特别是腹部皮肤伤口愈合的进展。为OA研究提供了一种实用可靠的动物模型。
BACKGROUND: This study aimed to establish an animal model of open abdomen (OA) through temporary abdominal closure via different techniques.
METHODS: Adult male Sprague-Dawley rats were randomly divided into three groups: group A (OA with polypropylene mesh alone); group B (OA with polypropylene mesh combined with a patch); and group C (OA with polypropylene mesh and a sutured patch). Vital signs, pathophysiological changes, and survival rates were closely monitored in the rats for 7 days after surgery. Abdominal X-rays and histopathological examinations were performed to assess abdominal organ changes and wound healing.
RESULTS: The results showed no significant difference in mortality rates among the three groups (p > 0.05). However, rats in group B exhibited superior overall condition, cleaner wounds, and a higher rate of wound healing compared to the other groups (p < 0.05). Abdominal X-rays indicated that varying degrees of distal intestinal obstruction in all groups. Histopathological examinations revealed fibrous hyperplasia, inflammatory cell infiltration, neovascularization, and collagen deposition in all groups. Group B demonstrated enhanced granulation tissue generation, neovascularization, and collagen deposition compared to the other groups (p < 0.05).
CONCLUSIONS: Polypropylene mesh combined with patches is the most suitable method for establishing an animal model of OA. This model successfully replicated the pathological and physiological changes in postoperative patients with OA, specifically the progress of abdominal skin wound healing. It provides a practical and reliable animal model for OA research.