■探讨不同OP材料条件下佩戴眼假体(OP)的眼科患者的细菌菌群变化和微生物组多样性。
■进行了横断面临床研究,包括19例OP患者和23例健康受试者。从上部收集样本,下睑,卡伯尔,和穹窿结膜.应用16SrRNA测序鉴定样品中的细菌菌群。通过问卷确定每位OP患者的眼睛舒适度。此外,还收集了每个参与者的人口统计信息。
■OP患者眼部菌群的多样性和丰富度明显高于健康受试者。植物种类分析的结果还表明,在OP患者中,致病性微生物如志贺氏菌和梭杆菌显著增加,而乳酸菌和乳球菌的常驻菌明显减少。在OP患者的自我比较中,与聚甲基丙烯酸甲酯(PMMA)相比,玻璃的假体材料将导致机会性病原体如产碱菌的定植增加,皮氏杆菌和螺旋藻,而性别和年龄对眼部菌群无显著影响。
■OP患者的眼部菌群与健康人明显不同。大量的病原微生物定植可能与OP患者眼部不适和眼部疾病有重要的潜在关系。PMMA,作为一种人造眼材料,在减少机会性病原体定植方面显示出潜在的优势。
To explore the changes of bacterial flora in anophthalmic patients wearing ocular prosthesis (OP) and the microbiome diversity in conditions of different OP materials.
A cross-sectional clinical study was conducted, involving 19 OP patients and 23 healthy subjects. Samples were collected from the upper, lower palpebral, caruncle, and fornix conjunctiva. 16S rRNA sequencing was applied to identify the bacterial flora in the samples. The eye comfort of each OP patient was determined by a questionnaire. In addition, demographics information of each participant was also collected.
The diversity and richness of ocular flora in OP patients were significantly higher than that in healthy subjects. The results of flora species analysis also indicated that in OP patients, pathogenic microorganisms such as Escherichia Shigella and Fusobacterium increased significantly, while the resident flora of Lactobacillus and Lactococcus decreased significantly. Within the self-comparison of OP patients, compared with Polymethyl Methacrylate (PMMA), prosthetic material of glass will lead to the increased colonization of opportunistic pathogens such as Alcaligenes, Dermabacter and Spirochaetes, while gender and age have no significant impact on ocular flora.
The ocular flora of OP patients was significantly different from that of healthy people. Abundant colonization of pathogenic microorganisms may have an important potential relationship with eye discomfort and eye diseases of OP patients. PMMA, as an artificial eye material, demonstrated potential advantages in reducing the colonization of opportunistic pathogens.