■近红外花青染料具有高灵敏度和空间分辨率的成像能力,但它们也有不可避免的缺点,如光漂白,低水溶性,荧光猝灭,和毒副作用。作为一种有效的生物药物载体,白蛋白与花青染料结合形成白蛋白@染料纳米颗粒。这些纳米颗粒可以缓解上述问题,并广泛用于肿瘤成像和光热治疗。
■这里,将新合成的近红外染料IR-817与牛血清白蛋白(BSA)结合,制备出BSA@IR-817纳米颗粒。通过荧光发射和吸收的检测,确定了BSA和IR-817的最佳浓度和比例。随后,动态光散射(DLS)测量和扫描电子显微镜(SEM)用于BSA@IR-817纳米粒子的物理表征。最后,进行了体外和体内实验以评估BSA@IR-817纳米颗粒的荧光成像和光热治疗潜力。
■IR-817通过共价缀合和超分子包封吸附到BSA载体上,导致分散的形成,同质,和稳定的纳米粒子,粒径范围为120-220nm。BSA@IR-817不只改良了水溶性差,荧光猝灭,IR-817的毒副作用,但也增强了近红外区的吸收和荧光发射峰,以及可见光谱中的荧光。此外,BSA@IR-817结合激光808照射能够在温度超过50°C的情况下将光能转化为热能。通过创建皮下黑色素瘤的小鼠模型,发现BSA@IR-817在激光照射后的肿瘤抑制率大于99%,并且它实现了几乎完全的肿瘤消融而没有引起明显的毒性。
■我们的研究,因此,提出使用安全有效的光热纳米粒子进行成像,诊断,和黑色素瘤的治疗,并为未来的生物医学应用提供了一个有前途的策略。
UNASSIGNED: Near-infrared cyanine dyes have high sensitivity and spatial resolution imaging capabilities, but they also have unavoidable drawbacks such as photobleaching, low water solubility, fluorescence quenching, and toxic side effects. As an effective biologic drug carrier, albumin combines with cyanine dyes to form albumin@dye nanoparticles. These nanoparticles can alleviate the aforementioned issues and are widely used in tumor imaging and photothermal therapy.
UNASSIGNED: Herein, a newly synthesized near-infrared dye IR-817 was combined with bovine serum albumin (BSA) to create BSA@IR-817 nanoparticles. Through the detection of fluorescence emission and absorption, the optimal concentration and ratio of BSA and IR-817 were determined. Subsequently, dynamic light scattering (DLS) measurements and scanning electron microscopy (SEM) were used for the physical characterization of the BSA@IR-817 nanoparticles. Finally, in vitro and in vivo experiments were conducted to assess the fluorescence imaging and photothermal therapeutic potential of BSA@IR-817 nanoparticles.
UNASSIGNED: IR-817 was adsorbed onto the BSA carrier by covalent conjugation and supramolecular encapsulation, resulting in the formation of dispersed, homogeneous, and stable nanoparticles with a particle size range of 120-220 nm. BSA@IR-817 not only improved the poor water solubility, fluorescence quenching, and toxic side effects of IR-817 but also enhanced the absorption and fluorescence emission peaks in the near-infrared region, as well as the fluorescence in the visible spectrum. In addition, BSA@IR-817 combined with laser 808 irradiation was able to convert light energy into heat energy with temperatures exceeding 50 °C. By creating a mouse model of subcutaneous melanoma, it was discovered that the tumor inhibition rate of BSA@IR-817 was greater than 99% after laser irradiation and that it achieved nearly complete tumor ablation without causing significant toxicity.
UNASSIGNED: Our research, therefore, proposes the use of safe and effective photothermal nanoparticles for the imaging, diagnosis, and treatment of melanoma, and offers a promising strategy for future biomedical applications.