%0 Journal Article
%T Construction of an effective near-infrared fluorescence "turn-on" probe for hydrogen sulfide detection and imaging in living inflammatory cell and zebrafish models.
%A Chen S
%A Liu T
%A Yuan X
%A Zhou L
%J Spectrochim Acta A Mol Biomol Spectrosc
%V 323
%N 0
%D 2024 Dec 15
%M 39068845
%F 4.831
%R 10.1016/j.saa.2024.124863
%X Hydrogen sulfide (H2S) can act as a gaseous signaling mediator closely associated with inflammation development. In this work, we designed a fluorescence turn-on near-infrared (NIR) fluorescent probe CIT-H2S based on Intermolecular Charge Transfer (ICT) for the detection of H2S in living inflammatory cells and zebrafish. On this basis, a dicyanoisophorone fluorophore was chosen as the fluorescence signal reporting group of CIT-H2S, and an azide group was constructed as the recognition group of H2S. CIT-H2S is characterized by high selectivity and sensitivity for H2S over other interference species. The fluorescence intensity at 661 nm showed good linearity in the range of H2S concentration from 0 to 10 μM, with an excellent limit of detection (LOD) as low as 81.52 nM. Impressively, CIT-H2S has been visualized for detecting H2S in drug-induced inflammatory cell and zebrafish models, thus indicating that CIT-H2S is a robust tool with the ability to study the occurrence and development of hydrogen sulfide and inflammation.