Abstract：
Hydrogen sulfide (H2S) can act as a gaseous signaling mediator closely associated with inflammation development. In this work, we designed a fluorescence turn-on near-infrared (NIR) fluorescent probe CIT-H2S based on Intermolecular Charge Transfer (ICT) for the detection of H2S in living inflammatory cells and zebrafish. On this basis, a dicyanoisophorone fluorophore was chosen as the fluorescence signal reporting group of CIT-H2S, and an azide group was constructed as the recognition group of H2S. CIT-H2S is characterized by high selectivity and sensitivity for H2S over other interference species. The fluorescence intensity at 661 nm showed good linearity in the range of H2S concentration from 0 to 10 μM, with an excellent limit of detection (LOD) as low as 81.52 nM. Impressively, CIT-H2S has been visualized for detecting H2S in drug-induced inflammatory cell and zebrafish models, thus indicating that CIT-H2S is a robust tool with the ability to study the occurrence and development of hydrogen sulfide and inflammation.
摘要：
硫化氢（H2S）可以作为与炎症发展密切相关的气体信号传导介质。在这项工作中,我们设计了一种基于分子间电荷转移（ICT）的荧光开启近红外（NIR）荧光探针T-H2S，用于检测活炎症细胞和斑马鱼中的H2S。在此基础上,选择双氰基异佛尔酮荧光团作为CIT-H2S的荧光信号报告基团，并构建了一个叠氮基团作为H2S的识别基团。ClT-H2S的特征在于对H2S相对于其他干扰物质的高选择性和灵敏度。661nm处的荧光强度在0～10μM的H2S浓度范围内呈良好的线性关系,具有优异的检测限(LOD),低至81.52nM。令人印象深刻的是,CIT-H2S已被可视化用于检测药物诱导的炎症细胞和斑马鱼模型中的H2S，因此表明CIT-H2S是一种强大的工具，能够研究硫化氢和炎症的发生和发展。
