石榴皮提取物(PPE),一种源自石榴的多酚化合物,因其抗氧化性能受到广泛关注,抗炎,和抑菌效果。研究了PPE对烧伤的潜在治疗作用,并探讨了其可能的机制。用PPE治疗二度烧伤的小型猪,静婉红,还有磺胺嘧啶银.进行苏木精-伊红(HE)染色以检测烧伤严重程度,然后在给药后第0、7、14、21和28天进行生物组织活检。免疫组织化学,westernblot,采用实时聚合酶链反应(RT-PCR)检测PPE处理后皮肤组织中VEGF-A和TGF-β1的蛋白和mRNA表达水平。此外,肉眼观察不同时间点皮肤创面愈合情况。他显示,经过28天的PPE治疗,皮肤组织形态明显改善。宏观数据监测表明,毛皮脱毛和生长时间缩短。同时,PPE治疗后伤口愈合率增加。免疫组织化学的组合,西方印迹,RT-PCR显示PPE治疗后,VEGF-A和TGF-β1的表达在第7天急剧增加,直到第14天保持高水平,在第21天显示下降趋势,在第28天接近正常水平。然而,在模型组中,烧伤后第28天VEGF-A和TGF-β1的蛋白和mRNA表达水平升高,这是一个缓慢的过程。结果表明,与模型组相比,VEGF-A和TGF-β1的峰值表达较早,这与脱壳是一致的,缩短毛皮生长时间,提高小型猪Ⅱ度烧伤模型创面愈合率。PPE对小型猪二度烧伤模型有显著的促进作用,这可能与VEGF-A和TGF-β1蛋白和基因表达水平的上调有关。
Pomegranate peel extract (PPE), a polyphenolic compound derived from pomegranate, has been widely concerned for its anti-oxidant, anti-inflammatory, and bacteriostatic effects. The potential therapeutic effect of PPE on burn injury was investigated, and its possible mechanisms were explored. Minipigs with second-degree burn were treated with PPE, Jing Wan Hong, and silver sulfadiazine. Hematoxylin-eosin (HE) staining was performed to detect burn severity, and then biological tissues were biopsied on days 0, 7, 14, 21, and 28 after administration. Immunohistochemistry, western blot, and real-time polymerase chain reaction (RT-PCR) were used to detect the protein and mRNA expression levels of VEGF-A and TGF-β1 in skin tissues after treatment with PPE. Furthermore, the skin wound healing at different time points was monitored by macroscopic observation. HE showed that after 28-day PPE treatment, the morphology of the skin tissue showed a significant improvement. Macroscopic data monitoring indicated that the decrustation and fur growing time was shortened. Meanwhile, the rate of wound healing increased after PPE treatment. The combination of immunohistochemistry, western blotting, and RT-PCR showed that after PPE treatment, expression of VEGF-A and TGF-β1 increased sharply on day 7, maintaining a high level until day 14, showing a downward trend on day 21, and approaching normal levels on day 28. However, in the model group, the protein and mRNA expression levels of VEGF-A and TGF-β1 increased on day 28 after burn injury, which was a slow process. Results indicated that compared with the model group, the peak expression level of VEGF-A and TGF-β1 was earlier, which was consistent with decrustation, shortening of fur growing time, and improvement of wound healing rate in
minipig second-degree burn model. PPE showed a significant promoting effect on
minipig second-degree burn model, which might be associated with the upregulation of the protein and gene expression levels of VEGF-A and TGF-β1.