■糖尿病前期,以一系列代谢异常为特征,增加糖尿病和心血管疾病的风险。汤之平(TZP),经过临床验证的中药配方,用于治疗葡萄糖耐量受损。然而,TZP干预糖尿病前期的潜在机制尚未完全阐明.
■本研究旨在评估TZP对糖尿病前期小鼠的保护作用并探讨其潜在机制。
■通过12周的高脂饮食(HFD)喂养建立糖尿病前期动物模型后,小鼠接受TZP治疗8周。与体重有关的各种参数,葡萄糖和脂质代谢,并测量胰岛素敏感性。组织病理学检查观察脂肪细胞大小和肝脏脂质沉积。SablePromethion系统评估了能量代谢活性。附睾白色脂肪组织(EWAT)的转录组学分析鉴定了富集的通路和基因。通过RT-PCR鉴定富集途径中的关键基因。
■我们的数据显示TZP的施用降低了糖尿病前期小鼠模型中的体重和脂肪量。TZP使葡萄糖和胰岛素水平正常化,改善胰岛素抵抗,血浆TC和FFA降低。通过病理检查证明TZP减轻了脂肪组织肥大和脂质沉积。间接量热法测量表明,TZP可能会增加VO2和EE水平。EWAT转录成果显示TZP逆转了与炎症和分解代谢代谢相干的通路和基因。RT-PCR显示炎症和脂解的mRNA表达,包括Tlr2,Ccr5,Ccl9,Itgb2,Lipe,Pnpla2,Cdo1,Ces1d,通过TZP处理改变了Echs1和Acad11。
■TZP有效缓解肥胖,糖脂代谢受损,和胰岛素抵抗。TZP的作用可能与功能失调的脂肪组织中基因表达的调节有关。
UNASSIGNED: Prediabetes, characterized by a series of metabolic abnormalities, increases the risk of diabetes and cardiovascular diseases. Tangzhiping (TZP), a clinically validated traditional Chinese medicine formula, is used to treat impaired glucose tolerance. However, the underlying mechanism of TZP in intervening prediabetes is not fully elucidated.
UNASSIGNED: The current study aimed to evaluate the protective effect of TZP against prediabetes mice and explore its potential mechanism.
UNASSIGNED: After establishing a prediabetic animal model through 12 weeks of high-fat diet (HFD) feeding, mice were subjected to TZP for 8 weeks. Various parameters related to body weight, glucose and lipid metabolism, and insulin sensitivity were measured. Histopathological examinations observed adipose cell size and liver lipid deposition. The Sable Promethion system assessed energy metabolism activity. Transcriptomic analysis of Epididymal white adipose tissue (EWAT) identified enriched pathways and genes. The key genes in the enriched pathways were identified through RT-PCR.
UNASSIGNED: Our data revealed that the administration of TZP reduced body weight and fat mass in a prediabetes mouse model. TZP normalized the glucose and insulin levels, improved insulin resistance, and decreased plasma TC and FFA. The alleviation of adipose tissue hypertrophy and lipid deposition by TZP was demonstrated through pathological examination. Indirect calorimetry measurements indicated a potential increase in VO2 and EE levels with TZP. The results of EWAT transcription showed that TZP reversed pathways and genes related to inflammation and catabolic metabolism. RT-PCR demonstrated that the mRNA expression of inflammation and
lipolysis, including Tlr2, Ccr5, Ccl9, Itgb2, Lipe, Pnpla2, Cdo1, Ces1d, Echs1, and Acad11, were changed by TZP treatment.
UNASSIGNED: TZP effectively alleviates obesity, impaired glucose and lipid metabolism, and insulin resistance. The effect of TZP might be associated with the regulation of gene expression in dysfunctional adipose tissue.