口腔鳞状细胞癌(OSCC)是口腔颌面部最常见的癌症。由于口腔特殊的生理解剖位置,这种疾病通常对咀嚼有重大影响,吞咽,语言,和患者的呼吸功能。近年来,随着医学分子生物学的发展,分子靶向治疗越来越受到临床重视,逐渐成为恶性肿瘤治疗的新方法。在这项研究中,具有高光热效应的金纳米星与搜索到的靶向抗体结合用于OSCC治疗。我们使用公共数据库中的数据集,通过加权基因共表达网络分析(WGCNA)构建基因共表达模块。发现绿松石模块和午夜蓝模块与肿瘤发生有最大的联系。利用Cytoscape软件对重要模块进行分析,选择每个模块的前10个基因;通过基因表达谱交互分析(GEPIA)对前10个基因进行生存分析,这表明这些基因(SERPINH1,MMP11,ADAM12,FADS3,SLC36A2,C1QTNF7,SCRG1和APOBEC2)作为与OSCC肿瘤发生相关的关键基因具有统计学意义。然后,选择靶向SERPINH1的抗SERPINH1抗体作为抑制剂,并与金纳米星联合用于光热辅助靶向治疗.因此,搜索到的关键基因可作为进一步精确诊断的生物标志物和治疗靶点。
Oral squamous cell carcinoma (OSCC) is the most common cancer in the oral and maxillofacial region. Due to the special physiological and anatomical position of the oral cavity, the disease often has a significant impact on the chewing, swallowing, language, and breathing functions of patients. In recent years, with the development of medical molecular biology, molecular targeted therapy has received increasing clinical attention and has gradually become a new method for the treatment of malignant tumors. In this research, gold nanostars with a high photothermal effect combined with the searched targeted antibody were used for OSCC therapy. We use the data set in the public database and construct a gene co-expression module by weighted gene co-expression network analysis (WGCNA). It was found that the turquoise module and the midnight blue module had the greatest connection to tumorigenesis. Cytoscape software was used to analyze the important modules, and the top 10 genes of each module were selected; the survival analysis of the top 10 genes was carried out by gene expression profiling interactive analysis (GEPIA), which indicated that these genes (SERPINH1, MMP11, ADAM12, FADS3, SLC36A2, C1QTNF7, SCRG1, and APOBEC2) have statistical significance as key genes that are related to the tumorigenesis of OSCC. Then, the anti-SERPINH1 antibody targeted to SERPINH1 was chosen as the inhibitor and combined with gold nanostars for photothermal assisted targeted therapy. Thus, the searched key genes can be regarded as biomarkers and therapeutic targets for further precise diagnosis.