Abstract：
Staphylococcus aureus contamination in food supplements poses substantial challenges to public health and large-scale production but the sensitive detection in a timely manner remains a bottleneck. Drawing inspiration from the sea hedgehog, gold nanostars (AuNSs) were leveraged to design an ultrasensitive surface-enhanced Raman scattering (SERS) biosensor for the determination of Staphylococcus aureus in food supplements. Besides the surface enhancement furnished by the AuNSs, Raman reporter molecules and specific aptamers sequentially self-assembled onto these AuNSs to construct the \"three-in-one\" SERS biosensor probe for label-based quantitation of Staphylococcus aureus. Following incubation with contaminated health product samples, the gold nanostars@Raman reporter-aptamer specifically recognize and assemble around Staphylococcus aureus cells, forming a distinctive sea hedgehog structure. This unique configuration results in an amplified Raman signal at 1338 cm-1 and an enhancement factor of up to 6.71 × 107. The entire quantitative detection process can be completed within 30 min, boasting an exceptional limit of detection as low as 1.0 CFU mL-1. The method exhibits a broad working range for the determination of Staphylococcus aureus, with concentrations spanning 2.15 CFU mL-1 to 2.15 × 105 CFU mL-1. Furthermore, it demonstrates outstanding precision, with relative standard deviation values consistently below 5.0%. As a showcase to validate the practicality of the SERS method, we conducted tests on determining Staphylococcus aureus in a herbal food supplement, i.e., Ginkgo Biloba extract (GBE); the results align closely with those obtained through the conventional lysogeny broth agar plate method, pointing to the potential applicability in real-world scenarios.
摘要：
食品补充剂中的金黄色葡萄球菌污染对公共卫生和大规模生产构成了重大挑战，但及时灵敏检测仍然是瓶颈。从海刺猬身上汲取灵感,利用金纳米星（AuNS）设计了一种超灵敏的表面增强拉曼散射（SERS）生物传感器，用于测定食品补充剂中的金黄色葡萄球菌。除了AuNS提供的表面增强外，拉曼报告分子和特异性适体顺序自组装到这些AuNS上，以构建“三合一”SERS生物传感器探针，用于基于标记的金黄色葡萄球菌定量。在与受污染的健康产品样品孵育后，金纳米星@拉曼报告适体专门识别和组装在金黄色葡萄球菌细胞周围，形成独特的海刺猬结构。这种独特的配置导致1338cm-1处的放大拉曼信号和高达6.71×107的增强因子。整个定量检测过程可在30min内完成，具有低至1.0CFUmL-1的特殊检测限。该方法对金黄色葡萄球菌的测定具有广泛的工作范围，浓度范围为2.15CFUmL-1至2.15×105CFUmL-1。此外，它展示了出色的精度，相对标准偏差值始终低于5.0%。作为验证SERS方法实用性的展示，我们进行了测定草药食品补充剂中金黄色葡萄球菌的测试，即，银杏叶提取物（GBE）；结果与通过常规溶生肉汤琼脂平板法获得的结果密切相关，指向现实世界场景中的潜在适用性。
