盐胁迫严重影响作物生长,导致作物质量和产量下降。应用外源物质提高作物的耐盐性,促进其在盐胁迫下的生长已成为一种广泛而有效的手段。丁香酚是一种植物来源的小分子,具有抗菌等药用特性,抗病毒,和抗氧化性能。在这项研究中,在存在或不存在丁香酚的情况下,将烟草幼苗置于含有NaCl的霍格兰溶液中,与应激耐受性相关的生理指标以及转录组测序。结果表明,丁香酚对盐胁迫下烟草幼苗的生长有促进作用。它促进碳和氮的代谢,增加硝酸还原酶(NR)的活性,蔗糖合酶(SS),谷氨酰胺合成酶(GS)分别为31.03、5.80和51.06%。它还激活了酶促和非酶促抗氧化系统,减少了烟草幼苗中活性氧的积累,并增加了超氧化物歧化酶(SOD)的活性,过氧化物酶(POD),过氧化氢酶(CAT),和抗坏血酸过氧化物酶(APX)24.38%,18.22%,21.60%,和28.8%,分别。谷胱甘肽(GSH)含量增加了29.49%,超氧阴离子(O2-)和丙二醛(MDA)含量分别降低29.83%和33.86%,分别。促进渗透调节,Na+含量减少34.34,K+含量增加41.25%,淀粉和可溶性糖分别增加了7.72%和25.42%,分别。它协调幼苗中的激素信号;脱落酸(ABA)和赤霉素3(GA3)的含量分别增加了51.93%和266.28%,分别。转录组数据表明差异表达基因主要富集在苯丙素生物合成中,MAPK信号通路,和植物激素信号转导途径。本研究结果揭示了丁香酚在调节植物抗性中的新作用,为利用外源物质缓解盐胁迫提供了参考。
Salt stress seriously affects crop growth, leading to a decline in crop quality and yield. Application of exogenous substances to improve the salt tolerance of crops and promote their growth under salt stress has become a widespread and effective means.
Eugenol is a small molecule of plant origin with medicinal properties such as antibacterial, antiviral, and antioxidant properties. In this study, tobacco seedlings were placed in Hoagland\'s solution containing NaCl in the presence or absence of
eugenol, and physiological indices related to stress tolerance were measured along with transcriptome sequencing. The results showed that
eugenol improved the growth of tobacco seedlings under salt stress. It promoted carbon and nitrogen metabolism, increased the activities of nitrate reductase (NR), sucrose synthase (SS), and glutamine synthetase (GS) by 31.03, 5.80, and 51.06%. It also activated the enzymatic and non-enzymatic antioxidant systems, reduced the accumulation of reactive oxygen species in the tobacco seedlings, and increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) by 24.38%, 18.22%, 21.60%, and 28.8%, respectively. The content of glutathione (GSH) was increased by 29.49%, and the content of superoxide anion (O2-) and malondialdehyde (MDA) were reduced by 29.83 and 33.86%, respectively. Promoted osmoregulation, the content of Na+ decreased by 34.34, K+ increased by 41.25%, and starch and soluble sugar increased by 7.72% and 25.42%, respectively. It coordinated hormone signaling in seedlings; the content of abscisic acid (ABA) and gibberellic acid 3 (GA3) increased by 51.93% and 266.28%, respectively. The transcriptome data indicated that the differentially expressed genes were mainly enriched in phenylpropanoid biosynthesis, the MAPK signaling pathway, and phytohormone signal transduction pathways. The results of this study revealed the novel role of
eugenol in regulating plant resistance and provided a reference for the use of exogenous substances to alleviate salt stress.