Abstract：
The high mortality rate of Singapore grouper iridovirus (SGIV) posing a serious threat to the grouper aquaculture industry and causing significant economic losses. Therefore, finding effective drugs against SGIV is of great significance. Eugenol (C10H12O2) is a phenolic aromatic compound, has been widely studied for its anti-inflammatory, antioxidant and antiviral capacity. In this study, we explored the effect of eugenol on SGIV infection and its possible mechanisms using grouper spleen cells (GS) as an in vitro model. We found that treatment of GS cells with 100 μM eugenol for 4 h exhibited the optimal inhibitory effect on SGIV. Eugenol was able to reduce the expression level of inflammatory factors by inhibiting the activation of MAPK pathway and also inhibited the activity of NF-κB and AP-1 promoter. On the other hand, eugenol attenuated cellular oxidative stress by reducing intracellular ROS and promoted the expression of interferon-related genes. Therefore, we conclude that eugenol inhibits SGIV infection by enhancing cellular immunity through its anti-inflammatory and antioxidant functions.
摘要：
新加坡石斑鱼虹彩病毒（SGIV）的高死亡率对石斑鱼养殖业构成严重威胁，并造成重大经济损失。因此,寻找有效的抗SGIV药物具有重要意义。丁香酚（C10H12O2）是一种酚类化合物，它的抗炎作用被广泛研究,抗氧化和抗病毒能力。在这项研究中,我们以石斑鱼脾细胞（GS）为体外模型，探讨了丁香酚对SGIV感染的影响及其可能的机制。我们发现用100μM丁香酚处理GS细胞4小时对SGIV表现出最佳的抑制作用。丁香酚能够通过抑制MAPK通路的激活而降低炎症因子的表达水平,同时抑制NF-κB和AP-1启动子的活性。另一方面,丁香酚通过减少细胞内ROS来减弱细胞氧化应激并促进干扰素相关基因的表达。因此,我们得出结论,丁香酚通过抗炎和抗氧化功能增强细胞免疫,从而抑制SGIV感染.
