未经证实:血小板反应蛋白-1(THBS1)来源于血小板,在血栓形成过程中作为促进血小板活化的重要止血介质。THBS1的遗传变异和mRNA表达与缺血性卒中(IS)的生物学联系值得基于人群的证据进一步验证。
UNASSIGNED:评估THBS1的单核苷酸多态性(SNPs)和mRNA表达与卒中后IS和长期死亡风险的关联。
UNASSIGNED:一项病例对照研究包括从江苏五家医院招募的4,584名IS患者,中国,和4,663个年龄性别匹配的对照,没有IS。一项队列研究招募了4,098名无卒中的参与者,从2009年持续到2022年。早期收集的3158例年龄在35至80岁之间的IS患者进行了平均5.86年的随访,以随访其长期死亡结果。THBS1基因的两个tagSNPs,在所有受试者中对rs2236471和rs3743125进行基因分型,并在314个IS病例和314个对照中使用RT-qPCR测量外周白细胞的THBS1mRNA表达。
UNASSIGNED:在IS病例和对照组之间,rs2236741和rs3743125的基因型和单倍型频率没有显着差异(均P>0.05)。此外,队列研究未观察到THBS1变异与IS发生率或IS后长期死亡风险之间存在显著关联(均P>0.05).IS病例中THBS1mRNA表达水平(2-ΔΔCT)与对照组大致相等(1.01vs.0.99,P=0.833)。此外,THBS1mRNA表达与全因死亡无显著相关性,中风死亡,IS患者死亡(均P>0.05)。
UNASSIGNED:因此,我们的研究提示,THBS1多态性和mRNA表达水平与IS风险和IS后长期死亡无显著关联.
UNASSIGNED: Thrombospondin-1 (THBS1) derived from platelets and acted as a critical mediator of hemostasis promoting platelet activation in thrombus formation. The biological connection of genetic variants and mRNA expression of THBS1 with ischemic stroke (IS) warrants further validation with population-based evidence.
UNASSIGNED: To evaluate the association of single nucleotide polymorphisms (SNPs) and mRNA expression of THBS1 with the risks of IS and long-term death after stroke.
UNASSIGNED: A case-control study consisted of 4,584 IS patients recruited from five hospitals in Jiangsu,
China, and 4,663 age-gender-matched controls free of IS. A cohort study enrolled 4,098 participants free of stroke and lasted from 2009 to 2022. Early collected 3158 IS patients aged between 35 and 80 years were followed up an average of 5.86-year to follow up their long-term death outcomes. Two tagSNPs of the THBS1 gene, rs2236471 and rs3743125, were genotyped in all subjects and THBS1 mRNA expression of peripheral leukocyte was measured using RT-qPCR in 314 IS cases and 314 controls.
UNASSIGNED: There is no significant difference in genotype and haplotype frequencies of rs2236741 and rs3743125 between IS cases and controls (all P > 0.05). Furthermore, the cohort studies did not observe significant associations between THBS1 variants and the risk of IS incidence or long-term death after IS (all P > 0.05). The THBS1 mRNA expression level (2-Δ Δ CT ) in IS cases was approximately equal to that in controls (1.01 vs. 0.99, P = 0.833). In addition, THBS1 mRNA expression had no significant association with all-cause death, stroke death, and IS death of IS patients (all P > 0.05).
UNASSIGNED: Therefore, our study suggested that there is no significant association of THBS1 polymorphisms and mRNA expression level with the risk of IS and long-term death after IS.