为了评估在废水处理中使用高NO2--N积累的好氧反硝化(AD)细菌进行脱氮的可能性,条件优化,以及涉及AD细菌的单一和混合氮源测试,Comamonassp.pw-6进行。结果表明,最佳碳源,pH值,C/N比,转速,该菌株的盐度被确定为琥珀酸盐,7,20,160转/分,0%,分别。Further,该菌株优先利用NH4+-N,NO3--N,和NO2--N,当NO3--N是它唯一的氮源时,92.28%的NO3--N(150mg·L-1)转化为NO2--N。然而,当NH4+-N和NO3--N构成混合氮源时,该菌株对NO3--N的利用率显著降低(p<0.05)。因此,提出了一种将pw-6细菌与传统的自养硝化相结合的策略,以实现pw-6细菌在含NH4-N废水处理中的应用。生物强化应用实验显示,NH4-N去除率(5.96±0.94mg·L-1·h-1)和NO3--N积累率(2.52±0.18mg·L-1·h-1)明显高于对照试验(p<0.05)。因此,具有高NO2--N积累的AD菌也可用于实际应用。为扩大废水处理用AD菌株的选择范围提供了依据。
To assess the possibility of using aerobic denitrification (AD) bacteria with high NO2--N accumulation for nitrogen removal in wastewater treatment, conditional optimization, as well as sole and mixed nitrogen source tests involving AD bacterium, Comamonas sp. pw-6 was performed. The results showed that the optimal carbon source, pH, C/N ratio, rotational speed, and salinity for this strain were determined to be succinate, 7, 20, 160 rpm, and 0%, respectively. Further, this strain preferentially utilized NH4+-N, NO3--N, and NO2--N, and when NO3--N was its sole nitrogen source, 92.28% of the NO3--N (150 mg·L-1) was converted to NO2--N. However, when NH4+-N and NO3--N constituted the mixed nitrogen source, NO3--N utilization by this strain was significantly lower (p < 0.05). Therefore, a strategy was proposed to combine pw-6 bacteria with traditional autotrophic nitrification to achieve the application of pw-6 bacteria in NH4+-N-containing wastewater treatment. Bioaugmented application experiments showed significantly higher NH4+-N removal (5.96 ± 0.94 mg·L-1·h-1) and lower NO3--N accumulation (2.52 ± 0.18 mg·L-1·h-1) rates (p < 0.05) than those observed for the control test. Thus, AD bacteria with high NO2--N accumulation can also be used for practical applications, providing a basis for expanding the selection range of AD strains for wastewater treatment.