Abstract：
The feasibility of a simultaneous nitrification, denitrification and fermentation process (SNDF) under electric stirrer agitation conditions was verified in a single reactor. Enhanced activated sludge for phenol degradation and denitrification in pharmaceutical phenol-containing wastewater under low dissolved oxygen conditions, additional inoculation with Comamonas sp. BGH and optimisation of co-metabolites were investigated. At a hydraulic residence time (HRT) of 28 h, 15 mg/L of substrate as strain BGH co-metabolised substrate degraded 650 ± 50 mg/L phenol almost completely and was accompanied by an incremental increase in the quantity of strain BGH. Strain BGH showed enhanced phenol degradation. Under trisodium citrate co-metabolism, strain BGH combined with activated sludge treated phenol wastewater and degraded NO2--N from 50 ± 5 to 0 mg/L in only 7 h. The removal efficiency of this group for phenol, chemical oxygen demand (COD) and TN was 99.67%, 90.25% and 98.71%, respectively, at an HRT of 32 h. The bioaugmentation effect not only promotes the degradation of pollutants, but also increases the abundance of dominant bacteria in activated sludge. Illumina MiSeq sequencing research showed that strain BGH promoted the growth of dominant genera (Acidaminobacter, Raineyella, Pseudarcobacter) and increased their relative abundance in the activated sludge system. These genera are resistant to toxicity and organic matter degradation. This paper provides some reference for the activated sludge to degrade high phenol pharmaceutical wastewater under the action of biological enhancement.
摘要：
同时硝化的可行性，在单个反应器中验证了在电搅拌器搅拌条件下的反硝化和发酵过程（SNDF）。强化活性污泥在低溶解氧条件下降解含酚制药废水中的苯酚和反硝化，用Comamonassp.额外接种。研究了BGH和共代谢物的优化。在水力停留时间(HRT)为28小时时，作为菌株BGH共代谢底物的15mg/L底物几乎完全降解650±50mg/L苯酚，并伴随着菌株BGH数量的增加。菌株BGH显示出增强的苯酚降解。在柠檬酸三钠共代谢下，菌株BGH与活性污泥结合处理苯酚废水，仅在7h内将NO2--N从50±5降解为0mg/L。化学需氧量(COD)和TN为99.67%,90.25%和98.71%，分别,在32小时的HRT。生物强化效应不仅促进污染物的降解，而且还增加了活性污泥中优势菌的丰度。IlluminaMiSeq测序研究表明，菌株BGH促进了优势属的生长（酸性不动杆菌，Raineyella,假杆菌）并增加了它们在活性污泥系统中的相对丰度。这些属对毒性和有机物降解具有抗性。本文为活性污泥在生物强化作用下降解高酚制药废水提供了一定的参考。
