背景:双孢蘑菇(A.外观完全白色的双孢蘑菇)受到消费者的欢迎。然而,双孢霉容易衰老和变质,导致褐变和氧化组织损伤,保质期有限。本研究调查了短波紫外线(UV-C,1.0kJm-2)对质量特征的辐照,酶促褐变,双孢菇在4°C下储存21d的防御性能。
结果:储存后,UV-C辐照的双孢菇在感官上质量有所提高,颜色,硬度,表现出较高的总酚含量,棕色黑色素前体,包括γ-谷氨酰胺-4-羟基苯(GHB),γ-谷氨酰胺-3,4-二羟基苯(GDHB)和酪氨酸含量,丙二醛(MDA)的积累降低,过氧化氢(H2O2),与对照样品相比,超氧自由基(O2•并保持膜完整性。此外,UV-C处理的双孢酵母显示出较低的褐变程度和较高的自我防御能力,这可能归因于超氧化物歧化酶(SOD)活性的增强。过氧化氢酶(CAT),苯丙氨酸解氨酶(PAL),几丁质酶(CHT),β-1,3葡聚糖酶活性(GLU)和抑制多酚氧化酶(PPO)的活性。
结论:这些结果表明,UV-C处理具有更高的自我防御能力可能是双孢蘑菇延缓衰老的机制。因此,建议将UV-C处理作为蘑菇的潜在实际应用。本文受版权保护。保留所有权利。
BACKGROUND: Agaricus bisporus with a completely white appearance is popular with consumers. However, A. bisporus is susceptible to senescence and spoilage, which results in browning and oxidative tissue damage, with limited shelf life. This study investigated the effects of shortwave ultraviolet (UV-C, 1.0 kJ m-2 ) irradiation on quality features, enzymatic browning, and defense properties of A. bisporus stored at 4 °C for 21 days.
RESULTS: After storage, UV-C irradiated A. bisporus had increased quality of sensory, color and hardness characteristics; it exhibited higher content of total phenolic, brown melanin precursors, including γ-glutaminyl-4-hydroxybenzene, γ-glutaminyl-3,4-dihydroxybenzene and tyrosine, lower accumulation of malondiadehyde, hydrogen peroxide and superoxide radical, and maintained membrane integrity in comparison to control samples. Besides, A. bisporus treated by UV-C showed lower degree of browning and higher level of self-defense capacity, which may be ascribed to the enhancement in activities of superoxide dismutase, catalase, phenylalanine ammonia lyase, chitinase and β-1,3-glucanase activity, while inhibiting polyphenol oxidase activity during storage.
CONCLUSIONS: These results indicate that the higher self-defense capacity with UV-C treatment might be the mechanism associated with the delay of senescence in Agaricus bisporus. Therefore, UV-C treatment is suggested as a potential practical application in mushrooms. © 2023 Society of Chemical Industry.