Abstract：
Postharvest rot of potato tubers caused by fungal pathogens is the main cause of significant economic losses, while also raising potential food safety issues. Integrated disease management, utilizing bio-safe and eco-friendly methods, represents a sustainable strategy for reducing postharvest losses in crops, including potato. In the current study, the application of the antagonistic yeast, Wickerhamomyces anomalus, combined with a UV-C treatment was evaluated for the management of postharvest Alternaria rot of potato tubers, caused by Alternaria tenuissima. Both W. anomalus and UV-C as individual treatments reduced the size of A. tenuissima infections on potato tubers, relative to the control, while the combined treatment of W. anomalus and UV-C exhibited the highest level of inhibition. W. anomalus or UV-C alone, and especially when used in combination, induced the expression of defense-related genes, including polyphenol oxidase, peroxidase, and β-1,3-glucanase, and also increased the level of flavonoids and lignin in potato tubers. Our findings indicate that the mechanism of action by which UV-C enhances the biocontrol effect of W. anomalus against postharvest Alternaria rot includes the activation of defense-related response in potato tubers. The integration of biocontrol agents and physical treatments (e.g., UV-C) represents an effective, eco-friendly hurdle technology for managing postharvest rot in potato.
摘要：
病原菌引起的马铃薯块茎采后腐烂是造成重大经济损失的主要原因,同时也引发了潜在的食品安全问题。综合疾病管理，利用生物安全和生态友好的方法，代表了减少作物采后损失的可持续战略，包括土豆.在目前的研究中,拮抗酵母的应用，反常Wickerhamomes,结合UV-C处理对马铃薯块茎采后链格孢菌腐烂的管理进行了评估，由变形虫引起的。W.anomalus和UV-C作为单独处理减少了马铃薯块茎上的A.tenuissima感染的大小，相对于控制，而W.反常和UV-C的联合治疗表现出最高的抑制水平。W.异常或UV-C单独，尤其是当组合使用时，诱导防御相关基因的表达，包括多酚氧化酶，过氧化物酶,和β-1,3-葡聚糖酶，还增加了马铃薯块茎中类黄酮和木质素的含量。我们的发现表明，UV-C增强W.anomalus对采后链格孢菌腐烂的生物防治作用的作用机制包括激活马铃薯块茎中与防御相关的反应。生物控制剂和物理治疗的整合(例如，UV-C)代表一种有效的，处理马铃薯采后腐烂的环保障碍技术。
