背景:临床组织粘合剂在处理紧急伤害方面仍然存在一些严重缺陷,如粘合强度不足和抗感染能力不足。在这里,一本小说,自我修复,抗菌羧甲基壳聚糖/聚醛葡聚糖(CMCS/PD)水凝胶被设计为急救组织粘合剂,用于有效的创伤急救管理。
方法:我们检查了凝胶形成时间,孔隙度,自我修复,抗菌性能,细胞毒性,粘合强度,和血液相容性。肝出血,尾巴遣散,并在体内构建大鼠皮肤伤口感染模型,分别。
结果:结果表明,CMCS/PD水凝胶具有快速凝胶形成(〜5s),良好的自我修复,和有效的抗菌能力,并能牢固地粘附在组织上(粘附强度为〜10kPa,破裂压力为327.5mmHg),具有优异的血液相容性和细胞相容性。这表明CMCS/PD水凝胶在充当创伤急诊处理的急救组织粘合剂方面具有广阔的前景。与商业止血凝胶(Surgiflo®)相比,观察到CMCS/PD水凝胶不仅实现了用于治愈肝出血和尾部切断的快速止血,而且与临床消毒剂凝胶(Prontosan®)相比,还表现出用于治疗急性皮肤创伤的优异抗感染。
结论:总体而言,CMCS/PD水凝胶为处理创伤紧急情况的急救组织粘合剂提供了有希望的候选药物.由于快速的凝胶形成时间,它也可以用作液体急救绷带,用于微创手术治疗。
BACKGROUND: Clinical tissue adhesives remain some critical drawbacks for managing emergency injuries, such as inadequate adhesive strength and insufficient anti-infection ability. Herein, a novel, self-healing, and antibacterial carboxymethyl chitosan/polyaldehyde dextran (CMCS/PD) hydrogel is designed as the first-aid tissue adhesive for effective trauma emergency management.
METHODS: We examined the gel-forming time, porosity, self-healing, antibacterial properties, cytotoxicity, adhesive strength, and hemocompatibility. Liver hemorrhage, tail severance, and skin wound infection models of rats are constructed in vivo, respectively.
RESULTS: Results demonstrate that the CMCS/PD hydrogel has the rapid gel-forming (~ 5 s), good self-healing, and effective antibacterial abilities, and could adhere to tissue firmly (adhesive strength of ~ 10 kPa and burst pressure of 327.5 mmHg) with excellent hemocompatibility and cytocompatibility. This suggests the great prospect of CMCS/PD hydrogel in acting as a first-aid tissue adhesive for trauma emergency management. The CMCS/PD hydrogel is observed to not only achieve rapid hemostasis for curing liver hemorrhage and tail severance in comparison to commercial hemostatic gel (Surgiflo ®) but also exhibit superior anti-infection for treating acute skin trauma compared with clinical disinfectant gel (Prontosan ®).
CONCLUSIONS: Overall, the CMCS/PD hydrogel offers a promising candidate for first-aid tissue adhesives to manage the trauma emergency. Because of the rapid gel-forming time, it could also be applied as a liquid first-aid bandage for mini-invasive surgical treatment.