目的:我们研究了索拉非尼(SFB)通过自然杀伤细胞来源的外泌体(NK-Exos)向乳腺癌球体的递送。方法:采用电穿孔法构建SFB-NK-Exos。它们的抗肿瘤作用通过甲基噻唑基四唑评估,吖啶橙/溴化乙锭,4\',6-二氨基-2-苯基吲哚,膜联蛋白/碘化丙啶,划痕和迁移测定,菌落形成,RT-PCR,蛋白质印迹和吸脂试验。结果:负荷有效率为46.66%。SFB-NK-Exos处理的球体显示出更高的细胞毒性效应(33%)和凋亡群体(44.9%)。尽管SFB-NK-Exos制剂中的SFB浓度降低,观察到与游离SFB相似的细胞毒性作用.细胞内贩运增加,药物的持续释放和选择性抑制作用证明了有效的导航。结论:这是SFB加载到NK-Exos的第一份报告,这导致了对癌细胞的显著细胞毒性增强。
这个总结是关于什么?这项研究描述了一种名为索拉非尼(SFB)的抗癌药物向实验室生长的球形癌细胞团的递送,称为球体。称为外泌体的碟状细胞结构被用作药物递送工具。这些外泌体由称为自然杀伤(NK)细胞的免疫细胞亚群产生。NK细胞负责杀死癌细胞。所以,这些外泌体与NK细胞具有相似的抗癌特性。我们想测试加载SFB的外泌体是否会有更好的抗癌效果。结果如何?使用不同的方法,将SFB装载在外泌体内并递送至球状体。结果表明,外泌体和SFB的组合可以提高靶向效果,减少对正常细胞的副作用,并允许药物的连续释放。在该处理后,球状体以更高的效力被杀死。这项研究的结果意味着什么?NK细胞来源的外泌体和SFB的组合可以导致更好的对癌细胞的细胞毒性。因此,与单用SFB治疗相比,该策略具有更好的抗癌效果.
Aim: We investigated the delivery of sorafenib (SFB) to breast cancer spheroids by natural killer cell-derived exosomes (NK-Exos). Methods: SFB-NK-Exos were constructed by electroporation. Their antitumor effects were evaluated by methyl thiazolyl tetrazolium, acridine orange/ethidium bromide, 4\',6-diamidino-2-phenylindole, annexin/propidium iodide, scratch and migration assay, colony formation, RT-PCR, western blot and lipophagy tests. Result: The loading efficacy was 46.66%. SFB-NK-Exos-treated spheroids showed higher cytotoxic effects (33%) and apoptotic population (44.9%). Despite the reduction of SFB concentration in the SFB-NK-Exos formulation, similar cytotoxic effects to those of free SFB were observed. Increased intracellular trafficking, sustained release of the drug and selective inhibitory effects demonstrated efficient navigation. Conclusion: This is the first report for SFB loading into NK-Exos, which led to significant cytotoxic intensification against cancer cells.
What is this summary about? This study describes the delivery of an anticancer drug called sorafenib (SFB) to laboratory-grown spherical masses of cancer cells called spheroids. Saucer-like cellular structures called exosomes were used as drug-delivery tools. These exosomes were produced by a subgroup of immune cells called natural killer (NK) cells. NK cells are responsible for killing cancer cells. So, these exosomes share similar anticancer properties with NK cells. We wanted to test whether exosomes loaded with SFB would have better anticancer effects. What were the results? Using different methods, SFB was loaded within the exosomes and delivered to the spheroids. The obtained results showed that a combination of exosomes and SFB could improve the targeting efficacy, reducing the side effects to the normal cells and allowing continuous release of the drug. The spheroids were killed with higher efficacy following this treatment. What do the results of the study mean? The combination of NK cell-derived exosomes and SFB could lead to better cytotoxicity against cancer cells. Therefore, this strategy could have better anticancer effects compared with SFB treatment alone.