Abstract：
In contrast to traditional two-dimensional cell-culture conditions, three-dimensional (3D) cell-culture models closely mimic complexin vivoconditions. However, constructing 3D cell culture models still faces challenges. In this paper, by using micro/nano fabrication method, including lithography, deposition, etching, and lift-off, we designed magnetic nanostructures resembling a crown of thorns. This magnetic crown of thorns (MCT) nanostructure enables the isolation of cells that have endocytosed magnetic particles. To assess the utility of this nanostructure, we used high-flux acquisition of Jurkat cells, an acute-leukemia cell line exhibiting the native phenotype, as an example. The novel structure enabled Jurkat cells to form spheroids within just 30 min by leveraging mild magnetic forces to bring together endocytosed magnetic particles. The size, volume, and arrangement of these spheroids were precisely regulated by the dimensions of the MCT nanostructure and the array configuration. The resulting magnetic cell clusters were uniform in size and reached saturation after 1400 s. Notably, these cell clusters could be easily separated from the MCT nanostructure through enzymatic digestion while maintaining their integrity. These clusters displayed a strong proliferation rate and survival capabilities, lasting for an impressive 96 h. Compared with existing 3D cell-culture models, the approach presented in this study offers the advantage of rapid formation of uniform spheroids that can mimicin vivomicroenvironments. These findings underscore the high potential of the MCT in cell-culture models and magnetic tissue enginerring.
摘要：
与传统的二维（2D）细胞培养条件相反，三维（3D）细胞培养模型密切模拟复杂的体内条件。然而,构建3D细胞培养模型仍然面临挑战。在本文中,通过使用微/纳米制造方法，包括光刻，沉积,蚀刻,和起飞，我们设计了类似荆棘冠的磁性纳米结构。这种磁性刺冠(MCT)纳米结构能够分离具有内吞磁性颗粒的细胞。为了评估这种纳米结构的实用性，我们使用了Jurkat细胞的高通量采集,表现出天然表型的急性白血病细胞系，作为一个例子。这种新颖的结构使Jurkat细胞能够在30分钟内通过利用温和的磁力将内吞的磁性颗粒聚集在一起而形成球体。大小,volume,这些球状体的排列由MCT纳米结构的尺寸和阵列配置精确地调节。得到的磁性细胞团簇大小均匀,在1400秒后达到饱和。值得注意的是,这些细胞簇可以很容易地通过酶消化与MCT纳米结构分离，同时保持其完整性。这些集群显示出强大的增殖速度和生存能力，令人印象深刻的96小时。与现有的3D细胞培养模型相比，本研究中提出的方法提供了快速形成可以模拟体内微环境的均匀球体的优势。这些发现强调了MCT在细胞培养模型和磁性组织接合中的高潜力。
