背景:以前,基于毛细管电泳平台构建了一个新的64个基因座的多重系统,包括59个常染色体插入/缺失(A-InDels),两个Y染色体InDels,两个迷你短串联重复(miniSTR),和Amelogenin基因.这项研究的目的是评估这种多重系统对个体识别的效率,中国贺州汉族(CHH)和湖北土家族(CTH)群体的亲子鉴定和生物地理血统推断,为法医人类学和人口遗传学研究提供有价值的见解。
结果:59个A-InDels和两个miniSTR的辨别力(CDP)和排除概率(CPE)的累积值为0.999999999999999999999999999999754,0.9999999905;CTH和CHH组中的0.99999999999999999999998,0.999999999898,分别。当似然比阈值设置为1或10时,可以从无关的个体对中识别出超过95%的完整兄弟对。CTH组和CHH组假阳性率均低于1.2%。利用三种算法构建了基于35个种群的生物地理祖先推理模型:随机森林,自适应增强和极端梯度增强,然后应用10倍交叉验证分析来测试这三个模型,平均准确率为86.59%,84.22%和87.80%,分别。此外,我们还使用FST的群体统计方法调查了两个研究组与33个参考群体之间的遗传关系,DA,系统发育树,PCA,结构和TreeMix分析。目前的结果表明,与其他大陆人口相比,CTH和CHH组与东亚人群的遗传亲和力较高.
结论:该新型多重系统在CTH和CHH组中具有较高的CDP和CPE,它可以用作个人识别和亲子鉴定的有力工具。根据各种遗传分析方法,CTH和CHH群体的遗传结构与参考东亚群体相对相似。
BACKGROUND: Previously, a novel multiplex system of 64 loci was constructed based on capillary electrophoresis platform, including 59 autosomal insertion/deletions (A-InDels), two Y-chromosome InDels, two mini short tandem repeats (miniSTRs), and an Amelogenin gene. The aim of this study is to evaluate the efficiencies of this multiplex system for individual identification, paternity testing and biogeographic ancestry inference in Chinese Hezhou Han (CHH) and Hubei Tujia (CTH) groups, providing valuable insights for forensic anthropology and population genetics research.
RESULTS: The cumulative values of power of discrimination (CDP) and probability of exclusion (CPE) for the 59 A-InDels and two miniSTRs were 0.99999999999999999999999999754, 0.99999905; and 0.99999999999999999999999999998, 0.99999898 in CTH and CHH groups, respectively. When the likelihood ratio thresholds were set to 1 or 10, more than 95% of the full sibling pairs could be identified from unrelated individual pairs, and the false positive rates were less than 1.2% in both CTH and CHH groups. Biogeographic ancestry inference models based on 35 populations were constructed with three algorithms: random forest, adaptive boosting and extreme gradient boosting, and then 10-fold cross-validation analyses were applied to test these three models with the average accuracies of 86.59%, 84.22% and 87.80%, respectively. In addition, we also investigated the genetic relationships between the two studied groups with 33 reference populations using population statistical methods of FST, DA, phylogenetic tree, PCA, STRUCTURE and TreeMix analyses. The present results showed that compared to other continental populations, the CTH and CHH groups had closer genetic affinities to East Asian populations.
CONCLUSIONS: This novel multiplex system has high CDP and CPE in CTH and CHH groups, which can be used as a powerful tool for individual identification and paternity testing. According to various genetic analysis methods, the genetic structures of CTH and CHH groups are relatively similar to the reference East Asian populations.