膳食蛋白质通过不清楚的机制调节食物摄入(FI)。一种可能性是更高的蛋白质导致更多的摄食后热量产生(特定动态作用:SDA),导致更早的进餐终止(饱腹感增加),和抑制进一步摄入(增加饱腹感)。使用自动FI监测系统(BioDAQ)测试了饮食蛋白对C57BL/6J小鼠摄食行为的影响,同时体温(Tb)。总FI,进餐间隔(IMI,饱腹感)和餐量(MS,饱腹感)与低消费后Tb的变化有关(5%,LP),中等(15%,MP)和高(30%,HP)蛋白质饮食。在三个条件下测试饮食:1)室温(RT,21±1°C),2)室温和运行轮(RTRW)和3)低温(10°C)和运行轮(LTRW)。还使用混合模型比较了饮食和条件之间的差异。RT喂养HP饮食的小鼠,与LP和MP相比,由于提前终止膳食(饱腹效应),总FI降低。在RTRW条件下FI降低,饮食之间没有差异。在LTRW条件下,所有饮食的FI均显着增加,蛋白质含量导致较早的进餐终止(饱腹感),但不是进食之间的间隔(饱腹感)。在所有条件下喂食后Tb立即下降。尽管喂食HP的小鼠的总FI降低,通过增加饱腹度来介导,这种影响与进餐时Tb的增加无关.我们得出结论,膳食蛋白质对摄入的影响不是通过SDA和Tb介导的。
Dietary protein modulates food intake (FI) via unclear mechanism(s). One possibility is that higher protein leads to greater post-ingestive heat production (Specific dynamic action: SDA) leading to earlier meal termination (increased
satiation), and inhibition of further intake (increased satiety). The influence of dietary protein on feeding behaviour in C57BL/6J mice was tested using an automated FI monitoring system (BioDAQ), simultaneous to body temperature (Tb). Total FI, inter meal intervals (IMI, satiety) and meal size (MS,
satiation) were related to changes in Tb after consuming low (5%, LP), moderate (15%, MP) and high (30%, HP) protein diets. Diets were tested over three conditions: 1) room temperature (RT, 21 ± 1 °C), 2) room temperature and running wheels (RTRW) and 3) low temperature (10 °C) and running wheels (LTRW). The differences between diets and conditions were also compared using mixed models. Mice housed at RT fed HP diet, reduced total FI compared with LP and MP due to earlier meal termination (
satiation effect). FI was lowered in RTRW conditions with no differences between diets. FI significantly increased under LTRW conditions for all diets, with protein content leading to earlier meal termination (
satiation) but not the intervals between feeding bouts (satiety). Tb fell immediately after feeding in all conditions. Despite a reduction in total FI in mice fed HP, mediated via increased
satiation, this effect was not linked to increased Tb during meals. We conclude effects of dietary protein on intake are not mediated via SDA and Tb.