生物标志物的检测对医学诊断具有重要意义,食品安全,环境监测,和农业。然而,目前的生物检测技术往往需要复杂的仪器,昂贵的试剂,专业知识,和延长的程序,这使得满足快速需求变得具有挑战性,敏感,用户友好,经济测试。相比之下,横向流条(LFS)技术提供了简单的,快,和视觉上可访问的检测模式,实时分析临床标本,从而在各个领域找到了广泛的效用。在LFS领域,适体作为分子识别探针的应用与抗体相比具有明显的优势,包括成本效益,尺寸较小,易于合成,和化学稳定性。近年来,基于适体的LFS在定性、半定量,以及食品安全的定量检测,环境监测,临床诊断,和其他领域。这篇综述简要概述了不同的适体筛选方法,选择策略,基本原则,和程序,阐明他们各自的优势,局限性,和应用。此外,我们总结了基于适体的LFS的最新策略和机制,如三明治和竞争方法。此外,我们对基于适体构建的LFS进行了分类,考虑到这一领域的快速发展,并讨论了它们在生物和化学检测中的应用。最后,我们探讨了适体和基于适体的LFS发展的当前挑战和未来方向。尽管这次审查并不彻底,它将为了解基于适体的LFS的研究进展提供有价值的参考,并有助于开发新类型的适体传感器。
The detection of biomarkers is of great significance for medical diagnosis, food safety, environmental monitoring, and agriculture. However, bio-detection technology at present often necessitates complex instruments, expensive reagents, specialized expertise, and prolonged procedures, making it challenging to fulfill the demand for rapid, sensitive, user-friendly, and economical testing. In contrast, lateral flow strip (LFS) technology offers simple, fast, and visually accessible detection modality, allowing real-time analysis of clinical specimens, thus finding widespread utility across various domains. Within the realm of LFS, the application of aptamers as molecular recognition probes presents distinct advantages over antibodies, including cost-effectiveness, smaller size, ease of synthesis, and chemical stability. In recent years, aptamer-based LFS has found extensive application in qualitative, semi-quantitative, and quantitative detection across food safety, environmental surveillance, clinical diagnostics, and other domains. This review provided a concise overview of different aptamer screening methodologies, selection strategies, underlying principles, and procedural, elucidating their respective advantages, limitations, and applications. Additionally, we summarized recent strategies and mechanisms for aptamer-based LFS, such as the sandwich and competitive methods. Furthermore, we classified LFSs constructed based on aptamers, considering the rapid advancements in this area, and discussed their applications in biological and chemical detection. Finally, we delved into the current challenges and future directions in the development of aptamer and aptamer-based LFS. Although this review was not thoroughly, it would serve as a valuable reference for understanding the research progress of aptamer-based LFS and aid in the development of new types of aptasensors.