PDF(Pubmed)
Abstract：
Ofloxacin (OFL) is widely used in animal husbandry and aquaculture due to its low price and broad spectrum of bacterial inhibition, etc. However, it is difficult to degrade and is retained in animal-derived food products, which are hazardous to human health. In this study, a simple and efficient method was developed for the detection of OFL residues in meat products. OFL coupled with amino magnetic beads by an amination reaction was used as a stationary phase. Aptamer AWO-06, which showed high affinity and specificity for OFL, was screened using the exponential enrichment (SELEX) technique. A fluorescent biosensor was developed by using AWO-06 as a probe and graphene oxide (GO) as a quencher. The OFL detection results could be obtained within 6 min. The linear range was observed in the range of 10-300 nM of the OFL concentration, and the limit of the detection of the sensor was 0.61 nM. Furthermore, the biosensor was stored at room temperature for more than 2 months, and its performance did not change. The developed biosensor in this study is easy to operate and rapid in response, and it is suitable for on-site detection. This study provided a novel method for the detection of OFL residues in meat products.
摘要：
氧氟沙星(OFL)因其价格低廉、抑菌谱广,被广泛应用于畜牧业和水产养殖业,等。然而,它难以降解并保留在动物源性食品中，对人体健康有害。在这项研究中,建立了一种简单高效的检测肉制品中OFL残留的方法。通过胺化反应与氨基磁珠偶联的OFL用作固定相。适体AWO-06对OFL显示出高亲和力和特异性，使用指数富集(SELEX)技术进行筛选。通过使用AWO-06作为探针和氧化石墨烯（GO）作为猝灭剂开发了荧光生物传感器。OFL检测结果可在6min内获得。在10-300nM的OFL浓度范围内观察到线性范围，传感器的检测极限为0.61nM。此外，生物传感器在室温下储存超过2个月，它的表现没有改变。本研究开发的生物传感器操作简便、反应迅速,适用于现场检测。本研究为肉制品中OFL残留的检测提供了一种新方法。
