背景:大葱在中国也被命名为谢白。它是一种可食用的蔬菜,也是治疗冠心病的著名草药。AlliumchinenseG.Don(ACGD)和AlliummacrostemonBunge(AMB)是植物来源。这项研究的目的是探索心脏保护作用,和破译视觉空间分布和绝对含量的初级代谢产物从这两种草药。
方法:用H9c2细胞建立缺氧-复氧(H/R)诱导的心肌损伤模型。通过凋亡水平评估其保护作用。此外,进行了基质辅助激光解吸/电离飞行时间串联质谱成像方法(MALDI-TOFMSI),以显示包括脂肪酸在内的主要代谢物的空间位置,氨基酸,类胡萝卜素,这两种葱属草药中的维生素。应用多种分析方法通过液相色谱串联质谱法(LC-MS)对AMB和ACGD灯泡中的这些主要代谢物进行定量分析。
结果:首先,AMB和ACGD提取物均可增加H9c2细胞的细胞活力,并减轻H/R诱导的损伤。它们显著减少了细胞凋亡,伴随着BCL-2/BAX途径的激活。Further,基于MALDI-TOFMSI的相对定量结果显示几种氨基酸,脂肪酸,类胡萝卜素,新鲜鳞茎的外衣和鳞片中富含维生素,而一些初级代谢产物在其发育中的花蕾中含量丰富。绝对定量结果表明,ACGD球茎中氨基酸的总含量高于AMB,而脂肪酸和维生素的总含量在这两种葱属草药中提供了相反的趋势。类胡萝卜素和微量元素的总含量在AMB和ACGD样品之间没有显着差异。
结论:本研究将有助于了解这两种中药对心肌损伤的保护作用。及其主要养分的空间积累和定量含量水平。
BACKGROUND: Allii Macrostemonis Bulbus is also named Xiebai in
China. It is an edible vegetable, and also a famous herb for treating coronary heart disease. Allium chinense G. Don (ACGD) and Allium macrostemon Bunge (AMB) are it botanical sources. The aim of this study was to explore the cardioprotective effects, and decipher the visual spatial distribution and absolute content of primary metabolites derived from these two herbs.
METHODS: H9c2 cells were used to perform the hypoxia-reoxygenation (H/R)-induced myocardial injury model. Their protective effects were evaluated by apoptosis levels. Furthermore, matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry imaging approach (MALDI-TOF MSI) was carried out to present the spatial location of primary metabolites including fatty acids, amino acids, carotenoids, and vitamins in these two Allium herbs. Multiple analytical methods were applied to perform quantitative analysis of these primary metabolites in AMB and ACGD bulbs by liquid chromatography tandem mass spectrometry (LC-MS).
RESULTS: First, AMB and ACGD extracts both could increase the cell viability in H9c2 cells, and attenuate H/R-induced injury. They markedly decreased apoptosis, accompanied by activating the BCL-2/BAX pathway. Further, MALDI-TOF MSI-based relative
quantification results showed several amino acids, fatty acids, carotenoids, and vitamins were largely rich in the tunics and outside scales of fresh bulbs, while some primary metabolites were abundant in their developing flower buds. Absolute
quantification results displayed total contents of amino acids in ACGD bulbs were higher than those in AMB, while total contents of fatty acids and vitamins provides opposite trends in these two Allium herbs. The total contents of carotenoids and trace elements showed no significant differences between AMB and ACGD samples.
CONCLUSIONS: This study would be helpful to understand the myocardial injury protection effects of these two Allium herbs, and the spatial accumulation and quantitative content levels of their main nutrients.