Abstract：
Monitoring and quantifying ATP levels in vivo is essential to understanding its role as a signaling molecule in tumor progression and therapy. Nevertheless, the real-time monitoring and quantitative assessment of lysosomal ATP remains challenging due to the lack of accurate tools in deep tissues. In this study, based on the crosslinking enhanced emission (CEE) effect, we successfully synthesized red carbon dots (R-CDs) with dual emission properties for efficient quantification of intracellular ATP. The R-CDs emit in the near-infrared range and target lysosomes with rapid detection capabilities, rendering them exceptionally well-suited for directly observing and analyzing the dynamics of lysosomal ATP through live cell imaging techniques. Importantly, R-CDs have proven their efficacy in real-time monitoring of drug stimulus-induced fluctuations in endogenous lysosomal ATP concentration and have also been employed for quantifying and distinguishing lysosomal ATP levels among normal and cancer cell lines. These noteworthy findings emphasize the versatility of the R-CD as a valuable imaging tool for elucidating the functional role of lysosomal ATP in drug screening and cancer diagnostics and hold the promise of becoming a reference tool for deepening our understanding of drug mechanisms of action.
摘要：
体内监测和定量ATP水平对于理解其作为肿瘤进展和治疗中的信号分子的作用至关重要。然而，由于在深部组织中缺乏准确的工具,溶酶体ATP的实时监测和定量评估仍然具有挑战性.在这项研究中,基于交联增强发射(CEE)效应，我们成功合成了具有双重发射特性的红碳点（R-CD），用于有效定量细胞内ATP。R-CD在近红外范围内发射，并具有快速检测能力的目标溶酶体，使它们非常适合通过活细胞成像技术直接观察和分析溶酶体ATP的动力学。重要的是,R-CD已证明其在实时监测药物刺激诱导的内源性溶酶体ATP浓度波动中的功效，并且还用于定量和区分正常和癌细胞系之间的溶酶体ATP水平。这些值得注意的发现强调了R-CD作为一种有价值的成像工具的多功能性，用于阐明溶酶体ATP在药物筛选和癌症诊断中的功能作用，并有望成为加深我们对药物作用机制的理解的参考工具。
