Abstract：
Microplastics (MPs), especially polystyrene microplastics (PS-MPs), have emerged a new worldwide pollutant, prompting significant public concern regarding their detection in environmental media. Analysis of PS-MPs in soil remains as a challenging task for analysts due to the highly intricate matrices. This work presents a practical approach for detecting PS-MPs in soil, which involves dilute HCl-assisted extraction and gel permeation chromatography- ultraviolet detection (GPC-UV) analysis. The presence of MPs in soil was confirmed through the use of a scanning electron microscope in conjunction with energy dispersive spectroscopy investigation. PS-MPs was isolated from soil, by agitating it with a diluted HCl solution, filtering the resulting liquid, and dissolving the residue on the filter with THF. The extractant was subsequently determined by GPC-UV. The introduction of a small amount of HCl into the extraction system was found to greatly expedite the settling of soil in water and enhance the efficacy of extracting PS-MPs in about 30 min. The linear range of PS-MPs was from 1.0 to 100 μg/mL with R2 > 0.999. Good reproducibility was obtained with the intra-day relative standard deviation (RSD, n = 3) of 1.36 % and the inter-day RSD (n = 3) of 4.78 %. The concentration of PS-MPs in soil samples were N.D. - 2.33 μg/g, and the good recoveries were 76.7-100.3 %. The corresponding AFGEEprer score was calculated to be 0.59, indicating the concept of green analytical chemistry for the pretreatment method. These results indicated that this method has a powerful potential for the accurate and rapid determination of PS-MPs in soil.
摘要：
微塑料（MPs），特别是聚苯乙烯微塑料(PS-MPs)，出现了一种新的全球污染物，引起公众对他们在环境媒体中的检测的重大关注。由于矩阵非常复杂，土壤中PS-MPs的分析仍然是一项艰巨的任务。这项工作提出了一种检测土壤中PS-MPs的实用方法，其中涉及稀HCl辅助萃取和凝胶渗透色谱-紫外检测(GPC-UV)分析。通过使用扫描电子显微镜并结合能量色散光谱研究，证实了土壤中MP的存在。PS-MPs是从土壤中分离出来的,用稀释的HCl溶液搅拌，过滤产生的液体，用THF溶解过滤器上的残余物。随后通过GPC-UV测定萃取剂。发现在提取系统中引入少量HCl可以大大加快土壤在水中的沉降，并在约30分钟内提高提取PS-MPs的功效。PS-MPs的线性范围为1.0~100μg/mL,R2>0.999。用日内相对标准偏差(RSD，n=3)为1.36%,日间RSD(n=3)为4.78%。土壤样品中PS-MPs的浓度为N.D.-2.33μg/g，良好的回收率为76.7-100.3%。相应的AFGEEprer评分计算为0.59，表明预处理方法的绿色分析化学概念。这些结果表明，该方法具有准确，快速测定土壤中PS-MPs的强大潜力。
