植物生长促进根际细菌(PGPR)通过在自然气候下形成生物膜来提高作物产量并减少环境压力。最近,这些微生物基于生物膜的根定植已成为农业增强的有希望的策略。目前的工作旨在表征生物膜形成根际细菌对小麦生长和增产的影响。为此,从小麦根际中分离出天然根际细菌,并在无菌条件下表征了十种分离株的植物生长促进性状和生物膜产生。在这十个分离株中,根据植物生长促进性状的体外测定,确定了五个潜在的产生生物膜的PGPR。在受控和田间条件下进一步评估了它们对小麦生长和产量属性的影响。表面增强拉曼光谱分析进一步表明,所选细菌菌株产生的生物膜的生化组成包括蛋白质,碳水化合物,脂质,氨基酸,和核酸(DNA/RNA)。在生长室中接种的植物导致较大的根,射击,新鲜生物量比对照增加。同样,株高显著增加(13.3,16.7%),粮食产量(29.6%,17.5%),耕地面积(18.7,34.8%),氮含量(58.8,48.1%),在盆栽和田间试验中观察到谷物中的磷含量(63.0,51.0%),分别。通过16srRNA部分基因测序鉴定出两个最有前途的生物膜产生分离株为布鲁氏菌。(BF10),大芽孢杆菌(BF15)。此外,所有处理植物的叶片色素沉着和相对含水量均显着增加。一起来看,我们的结果表明,生物膜形成PGPR可以通过增强生长和生理反应来提高作物生产力,从而有助于可持续农业。
Plant growth-promoting rhizobacteria (PGPR) boost crop yields and reduce environmental pressures through biofilm formation in natural climates. Recently, biofilm-based root colonization by these microorganisms has emerged as a promising strategy for agricultural enhancement. The current work aims to characterize biofilm-forming rhizobacteria for wheat growth and yield enhancement. For this, native rhizobacteria were isolated from the wheat rhizosphere and ten isolates were characterized for plant growth promoting traits and biofilm production under axenic conditions. Among these ten isolates, five were identified as potential biofilm-producing PGPR based on in vitro assays for plant growth-promoting traits. These were further evaluated under controlled and field conditions for their impact on wheat growth and yield attributes. Surface-enhanced Raman spectroscopy analysis further indicated that the biochemical composition of the biofilm produced by the selected bacterial strains includes proteins, carbohydrates, lipids, amino acids, and nucleic acids (DNA/RNA). Inoculated plants in growth chamber resulted in larger roots, shoots, and increase in fresh biomass than controls. Similarly, significant increases in plant height (13.3, 16.7%), grain yield (29.6, 17.5%), number of tillers (18.7, 34.8%), nitrogen content (58.8, 48.1%), and phosphorus content (63.0, 51.0%) in grains were observed in both pot and field trials, respectively. The two most promising biofilm-producing isolates were identified through 16 s rRNA partial gene sequencing as Brucella sp. (BF10), Lysinibacillus macroides (BF15). Moreover, leaf pigmentation and relative water contents were significantly increased in all treated plants. Taken together, our results revealed that biofilm forming PGPR can boost crop productivity by enhancing growth and physiological responses and thus aid in sustainable agriculture.