为了表征和鉴定艾蒿不同部位的化学成分(根,茎,叶子,和种子),筛选出具有抗氧化活性的化合物。在这项研究中,超高效液相色谱-2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐-四极杆飞行时间串联质谱(UPLC-ABTS-Q-TOF-MS)作为在线组合技术。Porosell120SB-Aq(3.0mm×150mm,使用2.7μm)作为色谱柱,以乙腈(A)-0.2%甲酸水(B)为流动相进行梯度洗脱,并以正离子和负离子方式进行扫描。使用了MassLynx软件,并结合参考物质和相关文献,对阿吉不同部位的化学成分进行了鉴定和比较。采用在线检测系统对抗氧化活性成分进行检测,并通过清除效率对不同部位的活性成分的抗氧化活性进行了比较和评价。因此,从A.argyi不同部位的提取物中总共鉴定出87种化合物,从根中鉴定出38、72、85和33种成分,茎,叶子,和种子。筛选了22种具有抗氧化活性的化合物,并从根中鉴定出14、17、20和11种具有抗氧化活性的化合物,茎,叶子,和种子。结果表明,不同部位的艾叶化学成分和抗氧化成分存在一定的差异,为阿吉的资源利用和进一步的研究开发提供数据支持。
In order to characterize and identify the chemical components in different parts of Artemisia argyi(roots, stems, leaves, and seeds), compounds with antioxidant activity were screened. In this
study, ultra-performance liquid chromatography-2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt-quadrupole time-of-flight-tandem mass spectrometry(UPLC-ABTS-Q-TOF-MS) was used as an online combination technique. Poroshell 120 SB-Aq(3.0 mm×150 mm, 2.7 μm) was used as the column, and acetonitrile(A)-0.2% formic acid water(B) was adopted as the mobile phase to perform gradient elution and was scanned in positive and negative ion modes. MassLynx software was utilized, and combined with reference substances and related literature, the chemical components of different parts of A. argyi were identified and compared. The antioxidant active components were detected by using the online detection system, and the antioxidant activities of active components of different parts of A. argyi were compared and evaluated by scavenging efficiency. As a result, a total of 87 compounds were identified from extracts of different parts of A. argyi, and 38, 72, 85, and 33 components were identified from roots, stems, leaves, and seeds. 22 compounds with antioxidant activity were screened, and 14, 17, 20, and 11 compounds with antioxidant activity were identified from roots, stems, leaves, and seeds. The results show that there are certain differences in chemical components and antioxidant components of different parts of A. argyi, which provides data support for the resource utilization and further research and development of A. argyi.