■由活性氧(ROS)响应性药物递送系统介导的化疗可以潜在地减轻化疗药物的毒副作用并显着增强其治疗功效。然而,在肿瘤部位实现精确的靶向药物递送和对ROS响应性药物释放的实时控制仍然是一个艰巨的挑战。因此,本研究旨在描述一种具有特定肿瘤靶向能力的ROS反应性药物递送系统,用于在荧光(FL)和磁共振(MR)双峰成像指导下减轻化疗诱导的毒性,同时增强治疗效果.
■吲哚菁绿(ICG),阿霉素(DOX)前药pB-DOX和超顺磁性氧化铁(SPIO,通过双乳化方法将Fe3O4)包封在聚(乳酸-羟基乙酸共聚物)(PLGA)中,以制备ICG/pB-DOX/Fe3O4/PLGA纳米颗粒(IBFPNPs)。通过碳二亚胺方法用乳腺珠蛋白抗体(mAb)官能化IBFPNP的表面以构建靶向乳腺癌的mAb/IBFPNP(MIBFPNP)。此后,在体外和体内评估了MIBFPNP的FL和MR双峰成像能力。最后,研究了基于MIBFPNP的联合光动力疗法(PDT)和化疗疗效评估。
■多功能MIBFPNP对乳腺癌表现出显著的靶向功效。FL和MR双峰成像清楚地显示了体内靶向MIBFPNPs的分布。在近红外激光照射下,加载ICG的MIBFPNP有效地生成了PDT的ROS,实现精确的肿瘤消融。同时,它通过切割其敏感部分来触发pB-DOX的激活,从而恢复DOX活性并实现ROS反应性靶向化疗。此外,MIBFPNPs联合PDT和化疗提高双峰成像指导下的肿瘤消融效率。
■MIBFPNP构成了一种新型的双模态成像引导药物递送系统,用于靶向治疗乳腺癌,并提供精确和可控的联合治疗选择。
UNASSIGNED: Chemotherapy mediated by Reactive oxygen species (ROS)-responsive drug delivery systems can potentially mitigate the toxic side effects of chemotherapeutic drugs and significantly enhance their therapeutic efficacy. However, achieving precise targeted drug delivery and real-time control of ROS-responsive drug release at tumor sites remains a formidable challenge. Therefore, this study aimed to describe a ROS-responsive drug delivery system with specific tumor targeting capabilities for mitigating chemotherapy-induced toxicity while enhancing therapeutic efficacy under guidance of Fluorescence (FL) and Magnetic resonance (MR) bimodal imaging.
UNASSIGNED: Indocyanine green (ICG), Doxorubicin (DOX) prodrug pB-DOX and Superparamagnetic iron oxide (SPIO, Fe3O4) were encapsulated in poly(lactic-co-glycolic acid) (PLGA) by double emulsification method to prepare ICG/ pB-DOX/ Fe3O4/ PLGA nanoparticles (IBFP NPs). The surface of IBFP NPs was functionalized with mammaglobin antibodies (mAbs) by carbodiimide method to construct the breast cancer-targeting mAbs/ IBFP NPs (MIBFP NPs). Thereafter, FL and MR bimodal imaging ability of MIBFP NPs was evaluated in vitro and in vivo. Finally, the combined photodynamic therapy (PDT) and chemotherapy efficacy evaluation based on MIBFP NPs was studied.
UNASSIGNED: The multifunctional MIBFP NPs exhibited significant targeting efficacy for breast cancer. FL and MR bimodal imaging clearly displayed the distribution of the targeting MIBFP NPs in vivo. Upon near-infrared laser irradiation, the MIBFP NPs loaded with ICG effectively generated ROS for PDT, enabling precise tumor ablation. Simultaneously, it triggered activation of the pB-DOX by cleaving its sensitive moiety, thereby restoring DOX activity and achieving ROS-responsive targeted chemotherapy. Furthermore, the MIBFP NPs combined PDT and chemotherapy to enhance the efficiency of tumor ablation under guidance of bimodal imaging.
UNASSIGNED: MIBFP NPs constitute a novel dual-modality imaging-guided drug delivery system for targeted breast cancer therapy and offer precise and controlled combined treatment options.